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Titolo:
Modulation of kainate-induced responses by pentobarbitone and GYKI-53784 in rat abducens motoneurons in vivo
Autore:
Ruiz, A; Durand, J;
Indirizzi:
CNRS, UPR 9041, Unite Neurocybernet Cellulaire, F-13009 Marseille, France CNRS Marseille France F-13009 rnet Cellulaire, F-13009 Marseille, France
Titolo Testata:
BRAIN RESEARCH
fascicolo: 2, volume: 818, anno: 1999,
pagine: 421 - 430
SICI:
0006-8993(19990213)818:2<421:MOKRBP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMPA RECEPTORS; GLUTAMATE-RECEPTOR; SYNAPTIC TRANSMISSION; IN-VIVO; NMDA; NEURONS; 2,3-BENZODIAZEPINES; CYCLOTHIAZIDE; ACTIVATION; SUBUNIT;
Keywords:
AMPA; barbiturate anesthesia; 2,3-benzodiazepine; excitatory amino acid; kainate; ketamine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Durand, J CNRS,09PR 9041, Unite Neurocybernet Cellulaire, 280 Bd St Marguerite, F-130 CNRS 280 Bd St Marguerite Marseille France F-13009 erite, F-130
Citazione:
A. Ruiz e J. Durand, "Modulation of kainate-induced responses by pentobarbitone and GYKI-53784 in rat abducens motoneurons in vivo", BRAIN RES, 818(2), 1999, pp. 421-430

Abstract

The modulation of kainate-induced responses by pentobarbitone and the 2,3-benzodiazepine GYKI-53784 (LY303070), a potent non-competitive AMPA antagonist, was studied in vivo using both extracellular recordings of antidromic field potentials and intracellular recordings from abducens motoneurons in ketamine/diazepam-anesthetized rats. In previous studies on pentobarbitone-anesthetized rats [M. Ouardouz, J. Durand, GYKI-52466 antagonizes glutamateresponses but not NMDA and kainate responses in rat abducens motoneurons, Neurosci. Lett. 125 (1991) 5-8; M. Ouardouz, J. Durand, Involvement of AMPAreceptors in trigeminal postsynaptic potentials recorded in rat abducens motoneurons in vivo, fur. J. Neurosci. 6 (1994) 1662-1668; A. Ruiz, J. Durand, Blocking the trigeminal EPSPs in rat abducens motoneurons in vivo with the AMPA antagonists, NBQX and GYKI-53655, J. Neurophysiol. (1998) submitted], we showed that 2,3-benzodiazepines do not affect kainate-induced depolarizations in abducens motoneurons. Here, we tested whether pentobarbitone isinvolved in the pharmacological discrimination by 2,3-benzodiazepines between AMPA- and kainate-induced responses. Kainate-induced depolarizations were reversibly depressed after application of either GYKI-53784 and pentobarbitone. However, kainate-induced depolarizations were not inhibited by GYKI-53784 with pentobarbitone; they were even potentiated sometimes. Using extracellular recordings, we confirmed that in the presence of pentobarbitone,GYKI-53784 counteracts the effects of AMPA but not of kainate on antidromic field potentials in the abducens nucleus. Blockade of kainate-induced responses by GYKI-53784 was reversed with pentobarbitone, which appears relevant to the discrimination between AMPA- and kainate receptor-mediated responses in vivo. In the presence of pentobarbitone, kainate would depolarize motoneurons mainly via kainate receptors since kainate-induced responses werenot depressed by 2,3-benzodiazepines. This finding strongly favors the existence of kainate receptors in adult motoneurons but their role is still unknown. (C) 1999 Elsevier Science B.V. All rights reserved.

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Documento generato il 04/07/20 alle ore 20:35:31