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Titolo:
The proteasome regulates caspase-dependent and caspase-independent protease cascades during apoptosis of MO7e hematopoietic progenitor cells
Autore:
Wu, LW; Reid, S; Ritchie, A; Broxmeyer, HE; Donner, DB;
Indirizzi:
Indiana Univ, Sch Med, Walther Oncol Ctr, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 ol Ctr, Indianapolis, IN 46202 USA Indiana Univ, Sch Med, Dept Immunol & Microbiol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 robiol, Indianapolis, IN 46202 USA Walther Canc Inst, Indianapolis, IN 46208 USA Walther Canc Inst Indianapolis IN USA 46208 t, Indianapolis, IN 46208 USA
Titolo Testata:
BLOOD CELLS MOLECULES AND DISEASES
fascicolo: 1, volume: 25, anno: 1999,
pagine: 20 - 29
SICI:
1079-9796(19990115)25:1<20:TPRCAC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
STRAND-BREAK-REPAIR; SYMPATHETIC NEURONS; DNA FRAGMENTATION; DEATH; INHIBITORS; KINASE; LINE; INDUCTION; SUBSTRATE; PATHWAY;
Keywords:
DNA fragmentation; factor withdrawal; cell death;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Donner, DB IndianaNUniv, Sch Med, Walther Oncol Ctr, 1044 W Walnut St, Indianapolis, I Indiana Univ 1044 W Walnut St Indianapolis IN USA 46202 olis, I
Citazione:
L.W. Wu et al., "The proteasome regulates caspase-dependent and caspase-independent protease cascades during apoptosis of MO7e hematopoietic progenitor cells", BL CELL M D, 25(1), 1999, pp. 20-29

Abstract

Withdrawal of trophic support from growth factor-dependent MO7e human myeloid progenitor cells induces apoptosis characterized by DNA fragmentation and degradation of the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs). Inhibitors of caspase (ICE) protease family members did not inhibit apoptosis or DNA fragmentation induced by factor withdrawal, but blocked degradation of DNA-PKcs. Thus, caspase activity accounts for only a component of the apoptotic program in MO7e hematopoietic cells. The protease inhibitor TPCK, but not other protease inhibitors, blocked DNA fragmentation, but not degradation of DNA-PKcs during apoptosis of MO7e cells. Thus, caspase-independent and caspase-dependent protease cascades mediate distinct features of MO7e cell apoptosis. The proteasome inhibitors calpain inhibitor I and lactacystin promoted DNA fragmentation, degradation of DNA-PKcs and apoptosis of MO7e cells. The ability of lactacystin to promote DNA fragmentation was abrogated by TPCK, but not by caspase inhibitors, whereas the abilityof lactacystin to promote degradation of DNA-PKcs was blocked by caspase inhibitors, but not by TPCK, Thus, caspase-dependent and caspase-independentprotease cascades are downstream of and regulated by the proteasome, whichplays a central role in regulating the multiple protease cascades that induce apoptosis. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 12:17:53