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Titolo:
Differential distribution of N-methyl-D-aspartate receptor-2 subunit messenger RNA in the rat superior olivary complex
Autore:
Sato, K; Nakagawa, H; Kuriyama, H; Altschuler, RA;
Indirizzi:
Univ Michigan, Kresge Hearing Res Inst, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 ng Res Inst, Ann Arbor, MI 48109 USA
Titolo Testata:
NEUROSCIENCE
fascicolo: 3, volume: 89, anno: 1999,
pagine: 839 - 853
SICI:
0306-4522(199903)89:3<839:DDONRS>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
DENSITY PROTEIN PSD-95; AMINO-ACID RECEPTORS; NMDA RECEPTOR; GLUTAMATE RECEPTORS; COCHLEAR NUCLEUS; DISTINCT DISTRIBUTIONS; MOLECULAR DIVERSITY; NERVOUS-SYSTEM; BRAIN; EXPRESSION;
Keywords:
NMDA; receptor; superior olivary complex; glutamate; excitatory amino acid; auditory;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Altschuler, RA UnivUSAchigan, Kresge Hearing Res Inst, 1301 E Ann St, Ann Arbor, MI 48109 Univ Michigan 1301 E Ann St Ann Arbor MI USA 48109 MI 48109
Citazione:
K. Sato et al., "Differential distribution of N-methyl-D-aspartate receptor-2 subunit messenger RNA in the rat superior olivary complex", NEUROSCIENC, 89(3), 1999, pp. 839-853

Abstract

The distribution of N-methyl-D-aspartate receptor subunit messenger RNA was examined in the superior olivary complex of the rat using in situ hybridization methods. Non-radioactive methods showed N-methyl-D-aspartate receptor-1 and N-methyl-D-aspartate receptor-2A -2B and -2C subunit expression to be abundant in principal cells of all major superior olivary complex regions, with N-methyl-D-aspartate receptor-1 expression higher than N-methyl-D-aspartate receptor-2. After autoradiographic in situ hybridization, countingsilver grains over cells was used to quantitate and compare levels of expression of the N-methyl-D-aspartate receptor-2 subunits. N-Methyl-D-aspartate receptor-2A, -2B and -2C expression was detected over cells in the lateral and medial superior olive, the medial, lateral and ventral nucleus of thetrapezoid body, and the superior paraolivary nucleus. N-Methyl-D-aspartatereceptor-2D expression was only observed in the lateral superior olive, lateral nucleus of the trapezoid body and ventral nucleus of the trapezoid body. Three distinct patterns of labeling were observed. The lateral superiorolive and ventral nucleus of the trapezoid body showed higher expression of N-methyl-D-aspartate receptor-2A and -2C than other subunits; the medial nucleus of the trapezoid body showed higher N-methyl-D-aspartate receptor-2B expression than other subunits; and the medial superior olive, superior paraolivary nucleus and ventral nucleus of the trapezoid body showed relatively equivalent expression of N-methyl-D-aspartate receptor-2A, -2B and -2C subunits. N-Methyl-D-aspartate receptor-2D had the lowest expression, with levels greater than background in only the lateral superior olive, ventral nucleus of the trapezoid body and lateral nucleus of the trapezoid body. Immunocytochemistry using antibodies to N-methyl-D-aspartate receptor-2A, -2Band -2C showed immunolabeling consistent with in situ hybridization results. These results show diversity in expression of N-methyl-D-aspartate receptor-2 subunits in different superior olivary complex regions and would predict pharmacological differences. (C) 1999 IBRO. Published by Elsevier ScienceLtd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 09:06:07