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Titolo:
Crucial role of Fc gamma RIIa (CD32) in assessment of functional anti-Streptococcus pneumoniae antibody activity in human sera
Autore:
Rodriguez, ME; van der Pol, WL; Sanders, LAM; van de Winkel, JGJ;
Indirizzi:
Univ Utrecht Hosp, Dept Immunol, NL-3584 CX Utrecht, Netherlands Univ Utrecht Hosp Utrecht Netherlands NL-3584 CX CX Utrecht, Netherlands Univrlandsht Hosp, Dept Immunol & Medarex Europe, NL-3584 CX Utrecht, Nethe Univ Utrecht Hosp Utrecht Netherlands NL-3584 CX -3584 CX Utrecht, Nethe Univ Hosp Children Het Wilhelmina Kinderziekenhui, Utrecht, Netherlands Univ Hosp Children Het Wilhelmina Kinderziekenhui Utrecht Netherlands s Univ Nacl La Plata, Fac Ciencias Exactas, Ctr Invest & Desarrollo Fermentac Univ Nacl La Plata La Plata Buenos Aires Argentina Desarrollo Fermentac
Titolo Testata:
JOURNAL OF INFECTIOUS DISEASES
fascicolo: 2, volume: 179, anno: 1999,
pagine: 423 - 433
SICI:
0022-1899(199902)179:2<423:CROFGR>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PNEUMOCOCCAL POLYSACCHARIDE VACCINE; HUMAN-NEUTROPHILS; RECEPTOR-II; CONJUGATE VACCINE; IGG SUBCLASSES; PHAGOCYTOSIS; POLYMORPHISM; INFANTS; INFECTIONS; RESPONSES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: van de Winkel, JGJ Univecht,cht Hosp, Dept Immunol, G04-614,Heidelberglaan100, NL-3584 CX Utr Univ Utrecht Hosp G04-614,Heidelberglaan 100 Utrecht Netherlands NL-3584 CX
Citazione:
M.E. Rodriguez et al., "Crucial role of Fc gamma RIIa (CD32) in assessment of functional anti-Streptococcus pneumoniae antibody activity in human sera", J INFEC DIS, 179(2), 1999, pp. 423-433

Abstract

Immunoglobulin G (IgG)-mediated phagocytosis by polymorphonuclear leukocytes (PMNL) constitutes the main defense against Streptococcus pneumoniae, Two leukocyte IgG receptors, Fc gamma RIIa and Fc gamma RIIIb, are constitutively expressed on PMNL. Blocking experiments showed Fc gamma RIIa is crucial for opsonophagocytosis of serum-opsonized S. pneumoniae. The biallelic, genetically determined Fc gamma RIIa polymorphism (Fc gamma RIIa-R131 vs. IIa-H131) determines the capacity of IgG2-mediated phagocytosis via this receptor. Comparative studies with PMNL from donors either homozygous for Fc gamma RIIa-R131 or IIa-H131 showed the latter had higher phagocytic capacity. These results were confirmed in Fc gamma RIIa-R131- and Fc gamma RIIa-H131-transfected IIA1.6 cells. The performance of Fc gamma RIIa-transfected cells in S. pneumoniae phagocytosis was validated using sera from adults and children. Serum-induced phagocytic activity depended mainly on anti-pneumococcal IgG2 antibodies. Results obtained with PMNL and IIA1.6 cells showed high correlation (r = 0.94; P < .001), and support that Fc gamma RIIa transfectants are a good alternative to PMNL as effector cells in opsonophagocytosis assays.

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Documento generato il 29/09/20 alle ore 00:13:24