Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of doxorubicin, mitomycin C, and ethanol on Hep-G2 cells in vitro
Autore:
Castaneda, F; Kinne, RKH;
Indirizzi:
Max Planck Inst Mol Physiol, Abt Epithelphysiol, D-44026 Dortmund, GermanyMax Planck Inst Mol Physiol Dortmund Germany D-44026 6 Dortmund, Germany
Titolo Testata:
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
fascicolo: 1, volume: 125, anno: 1999,
pagine: 1 - 8
SICI:
0171-5216(199901)125:1<1:EODMCA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATOCELLULAR-CARCINOMA; INJECTION THERAPY; LIVER-CANCER; ABLATION THERAPY; HEPATIC-TUMORS; APOPTOSIS; ADRIAMYCIN; PHARMACOKINETICS; CHEMOTHERAPY; HEPATOCYTES;
Keywords:
cell proliferation; apoptosis; cell death; cultured cells; hepatocellular carcinoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Kinne, RKH Max Planck Inst Mol Physiol, Abt Epithelphysiol, Postfach 10 2664, D-44026 Max Planck Inst Mol Physiol Postfach 10 26 64 Dortmund Germany D-44026
Citazione:
F. Castaneda e R.K.H. Kinne, "Effects of doxorubicin, mitomycin C, and ethanol on Hep-G2 cells in vitro", J CANC RES, 125(1), 1999, pp. 1-8

Abstract

There are conflicting results for experiments aimed at determining whetheranticancer drug therapy of human hepatocellular carcinoma prolongs the survival rate effectively. The purpose of this study was to assess the effect of low concentrations of doxorubicin, mitomycin C, and ethanol on cell replication (cell number and proliferation), and cell apoptosis of cultured human hepatocellular carcinoma (Hep-G2) cells. After 1 day of exposure doxorubicin inhibited cell replication initially by 72%, but a partial recovery ofthe cell number was observed. Mitomycin C inhibited to the same extent butwithout recovery. Ethanol reduced the cell number even further, the maximum inhibition (12 days after exposure) being 96.4%. After 3 days of exposureall three agents stopped cell replication at a level of 2%-4% of the control (P < 0.001). Cell apoptosis was activated most strikingly by mitomycin C(5 mu g/ml) after 1 day of exposure and by ethanol (150 mu l/ml) after 3 days of exposure. Two-way repeated-measures analysis of variance showed statistically significant differences, with ethanol being the most significant followed by mitomycin C doxorubicin, and the control (P < 0.01). Thus, a low dose of ethanol combined with an exposure time of up to 3 days appears tobe an effective regimen to control growth of human hepatocellular carcinoma cells in vitro. The strong induction of apoptosis by ethanol might be of additional benefit for a local application in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 20:57:35