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Titolo:
Issue inhibitor of metalloproteinases-3 inhibits shedding of L-selectin from leukocytes
Autore:
Borland, G; Murphy, G; Ager, A;
Indirizzi:
Natl Inst Med Res, Div Cellular Immunol, London NW7 1AA, England Natl InstMed Res London England NW7 1AA mmunol, London NW7 1AA, England Univ E Anglia, Sch Biol Sci, Norwich NR4 7TJ, Norfolk, England Univ E Anglia Norwich Norfolk England NR4 7TJ h NR4 7TJ, Norfolk, England
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 5, volume: 274, anno: 1999,
pagine: 2810 - 2815
SICI:
0021-9258(19990129)274:5<2810:IIOMIS>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; ADHESION MOLECULE; TISSUE INHIBITOR; CELL-SURFACE; CHEMOTACTIC FACTORS; TERMINAL DOMAIN; MEL-14 ANTIGEN; IN-VITRO; IDENTIFICATION; DISINTEGRIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Ager, A Natl Inst Med Res, Div Cellular Immunol, Mill Hill,The Ridgeway, London NW7 Natl Inst Med Res Mill Hill,The Ridgeway London England NW7 1AA 7
Citazione:
G. Borland et al., "Issue inhibitor of metalloproteinases-3 inhibits shedding of L-selectin from leukocytes", J BIOL CHEM, 274(5), 1999, pp. 2810-2815

Abstract

Although the enzyme or enzymes mediating shedding of L-selectin have not yet been identified, this activity can be blocked by synthetic hydroxamic acid-based inhibitors of metalloproteinases such as Ro 31-9790, However, the endogenous matrix metalloproteinase inhibitor tissue inhibitor of metalloproteinases (TIMP)-1 does not block L-selectin shedding. Here, we report thatTIMP-3, but not TIMP-2, inhibits L-selectin shedding from mouse and human lymphocytes, Jurkat T cells, and human monocytes, TIMP-3 has an IC50 of 0.3-0.4 mu M on these cell types compared with 0.7-4.8 mu M for Ro 31-9790, A metalloproteinase (tumor necrosis factor-alpha (TNF-alpha)converting enzyme; ADAM17) has recently been identified which cleaves the pro-form of TNF-alpha to produce soluble cytokine, We compared inhibition of L-selectin shedding by TIMPs and Ro 31-9790 with inhibition of TNF-alpha shedding from human monocytes, TIMP-3 inhibited TNF-alpha shedding (IC50 of 0.1 mu M), as didRo 31-9790 (IC50 of 0.4 mu M). TIMP-2 had a partial effect, and TIMP-1 didnot inhibit. This study confirms that L-selectin sheddase is a metalloproteinase, but not a matrix metalloproteinase, and investigates the relationship between shedding of L-selectin and TNF-alpha.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:23:00