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Titolo:
Translational repression by human 4E-BP1 in yeast specifically requires human eIF4E as target
Autore:
Hughes, JMX; Ptushkina, M; Karim, MM; Koloteva, N; von der Haar, T; McCarthy, JEG;
Indirizzi:
UnivlManchester, Inst Sci & Technol, Dept Biomol Sci, Posttranscript Contro Univ Manchester Manchester Lancs England M60 1QD i, Posttranscript Contro
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 6, volume: 274, anno: 1999,
pagine: 3261 - 3264
SICI:
0021-9258(19990205)274:6<3261:TRBH4I>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
INITIATION-FACTOR EIF-4E; CAP-DEPENDENT TRANSLATION; SACCHAROMYCES-CEREVISIAE; MESSENGER-RNA; BINDING; SYSTEM; CELL; PHOSPHORYLATION; RIBOSOMES; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Hughes, JMX UnivlManchester, Inst Sci & Technol, Dept Biomol Sci, Posttranscript Contro Univ Manchester POB 88 Manchester Lancs England M60 1QD Contro
Citazione:
J.M.X. Hughes et al., "Translational repression by human 4E-BP1 in yeast specifically requires human eIF4E as target", J BIOL CHEM, 274(6), 1999, pp. 3261-3264

Abstract

4E-binding proteins (4E-BPs) are believed to have important regulatory functions in controlling the rate of translation initiation in mammalian cells. They do so by binding to the mRNA cap-binding protein, eIF4E, thereby inhibiting formation of the cap-binding complex, a process essential for cap-dependent translation initiation. We have reproduced the translation-repressive function of human 4E-BP1 in yeast and find its activity to be dependenton substitution of human eIF4E for its yeast counterpart. Translation initiation and growth are inhibited when human 4E-BP1 is expressed in a strain with the human eIF4E substitution, but not in an unmodified strain. We havecompared the relative affinities of human 4E-BP1 for human and yeast eIF4E, both in vitro using an m(7)GTP cap-binding assay and in vivo using a yeast two-hybrid assay, and find that the affinity of human 4E-BP1 for human eIF4E is markedly greater than for yeast eIF4E. Thus yeast eIF4E lacks structural features required for binding to human 4E-BP1. These results thereforedemonstrate that the features of eIF4E required for binding to 4E-BP1 are distinct from those required for cap-complex assembly.

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Documento generato il 20/09/20 alle ore 00:55:53