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Titolo:
PURINERGIC-MEDIATED INHIBITION OF NA-K+-ATPASE IN PROXIMAL TUBULE CELLS - ELEVATED CYTOSOLIC CA2+ IS NOT REQUIRED()
Autore:
JIN WW; HOPFER U;
Indirizzi:
CASE WESTERN RESERVE UNIV,SCH MED,DEPT PHYSIOL & BIOPHYS CLEVELAND OH44106 CASE WESTERN RESERVE UNIV,SCH MED,DEPT PHYSIOL & BIOPHYS CLEVELAND OH44106
Titolo Testata:
American journal of physiology. Cell physiology
fascicolo: 4, volume: 41, anno: 1997,
pagine: 1169 - 1177
SICI:
0363-6143(1997)41:4<1169:PIONIP>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; CANINE KIDNEY-CELLS; EXTRACELLULAR ATP; MULTIDRUG-RESISTANCE; ESSENTIAL-HYPERTENSION; LLC-PK1 CELLS; SALT INTAKE; NA+,K+-ATPASE; MECHANISMS; PATHWAY;
Keywords:
SODIUM-POTASSIUM-ADENOSINE-TRIPHOSPHATASE; ADENOSINE 5'-TRIPHOSPHATE; PURINERGIC RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
W.W. Jin e U. Hopfer, "PURINERGIC-MEDIATED INHIBITION OF NA-K+-ATPASE IN PROXIMAL TUBULE CELLS - ELEVATED CYTOSOLIC CA2+ IS NOT REQUIRED()", American journal of physiology. Cell physiology, 41(4), 1997, pp. 1169-1177

Abstract

The involvement of cytosolic Ca2+ concentration ([Ca2+](i)) as messenger for the regulation of Na+-K+-ATPase activity was investigated in arenal cell line recently developed by immortalization of early proximal tubule primary cultures from the Wistar-Kyoto rat strain. Na+-K+-ATPase was measured as short-circuit current (I-sc) in intact monolayersafter permeabilization of the apical plasma membrane with amphotericin B. With symmetrical solutions, I-sc quantitatively reflects Na+-K+-ATPase activity as judged by ouabain inhibition and dependence on Na+ and K+. Extracellular ATP (50%) effective concentration = 0.32 mM) on the apical side produced acute inhibition of Na+-K+-ATPase-generated I-sc of up to 50%. The inhibition peaked within 1 min and lasted similarto 5 min. The potency order was ATP > ADP >> beta,gamma-methyleneadenosine 5'-triphosphate UTP, consistent with a P-2y receptor. Extracellular ATP also stimulated a transient increase in [Ca2+](i). This increase had a similar time course as the inhibition of ATPase and reached apeak change of similar to 120 nM. However, the elevation of [Ca2+](i)is not required in the purinergic inhibition of the Na+-K+-ATPase, since, first, increases in [Ca2+](i) produced with a Ca2+ ionophore (ionomycin) failed to mimic the purinergic inhibition and, second, ,2-bis(2-aminophenoxy)ethane=N,N,N',N'-tetraacetic acid, which abolished the [Ca2+](i) elevation, failed to block the purinergic inhibition.

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Documento generato il 11/07/20 alle ore 15:49:56