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Titolo:
Glucose induces early growth response gene (Egr-1) expression in pancreatic beta cells
Autore:
Josefsen, K; Sorensen, LR; Buschard, K; Birkenbach, M;
Indirizzi:
Kommunehosp, Bartholin Inst, DK-1399 Copenhagen K, Denmark Kommunehosp Copenhagen Denmark K lin Inst, DK-1399 Copenhagen K, Denmark Univ Chicago, Majorie B Kovler Viral Oncol Lab, Chicago, IL 60637 USA UnivChicago Chicago IL USA 60637 Viral Oncol Lab, Chicago, IL 60637 USA
Titolo Testata:
DIABETOLOGIA
fascicolo: 2, volume: 42, anno: 1999,
pagine: 195 - 203
SICI:
0012-186X(199902)42:2<195:GIEGRG>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASE; MESSENGER-RNA STABILITY; IMMEDIATE EARLY GENES; COA CARBOXYLASE GENE; INSULIN GENE; TRANSCRIPTION FACTOR; MEMBRANE DEPOLARIZATION; TRANSLATIONAL CONTROL; TRANSPORTER ISOFORMS; PROMOTER ACTIVITY;
Keywords:
glucose stimulation; beta-cell line; MIN6; transcription factor; Egr-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Josefsen, K Kommunehosp, Bartholin Inst, DK-1399 Copenhagen K, Denmark Kommunehosp Copenhagen Denmark K -1399 Copenhagen K, Denmark
Citazione:
K. Josefsen et al., "Glucose induces early growth response gene (Egr-1) expression in pancreatic beta cells", DIABETOLOG, 42(2), 1999, pp. 195-203

Abstract

A copy deoxyribonucleic acid (cDNA) clone of the immediate early growth response gene, egr-1 (Krox-24, Zif268, NGFI-1), was isolated through subtractive hybridization screening to identify glucose-induced genes in pancreaticbeta cells. Glucose rapidly and transiently induced egr-1 mRNA in the SV40-transformed murine beta-cell line, MIN6. Glucose also increased egr-1 mRNAexpression in INS-1, beta TC3 and RINm5F beta-cell lines, although with different kinetics. Expression of the 82 kDa Egr-1 protein was induced both in MIN6 cells stimulated with glucose in vitro and in primary rat islet cells stimulated in vivo or in vitro. This response is unique to beta cells since glucose did not affect egr-1 expression in NIH-3T3 fibroblasts or glucose-sensitive hepatocytes. In beta cells egr-1 induction is specifically associated with insulin secretion, as it was not observed after stimulation with serum or insulin but was elicited by insulin secretagogues, including membrane depolarizing agents and cAMP agonists. Moreover, induction of egr-1 by glucose was inhibited by EDTA, indicating dependence on influx of extracellular Ca2+. Other immediate early response genes, c-fos and junB, were also induced following glucose stimulation with kinetics similar to egr-1, whereas c-jun and junD expression were not affected. Since the zinc-finger protein encoded by egr-1 is highly homologous to transcription factors that control expression of glucose-regulated genes in yeast, Egr-1 could mediate delayed adaptive responses of beta cells to sustained glucose stimulation through transcriptional regulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 06:37:52