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Titolo:
Cell lines of pulmonary and non-pulmonary origin as tools to study the effects of house dust mite proteinases on the regulation of epithelial permeability
Autore:
Winton, HL; Wan, H; Cannell, MB; Gruenert, DC; Thompson, PJ; Garrod, DR; Stewart, GA; Robinson, C;
Indirizzi:
St Georges Hosp, Sch Med, Dept Pharmacol & Clin Pharmacol, London SW17 0RE, St Georges Hosp London England SW17 0RE Clin Pharmacol, London SW17 0RE, UnivDeptif San Francisco, Cardiovasc Res Inst, UCSF Gene Therapy Core Ctr,Univ Calif San Francisco San Francisco CA USA 94143 ene Therapy Core Ctr, UnivDeptif San Francisco, Cardiovasc Res Inst, UCSF Gene Therapy Core Ctr,Univ Calif San Francisco San Francisco CA USA 94143 ene Therapy Core Ctr, Univ9,estern Australia, Queen Elizabeth II Med Ctr, Dept Med, Perth, WA 600 Univ Western Australia Perth WA Australia 6009 r, Dept Med, Perth, WA 600 Univ Manchester, Sch Biol Sci, Manchester, Lancs, England Univ ManchesterManchester Lancs England Sci, Manchester, Lancs, England UnivWAestern Australia, Queen Elizabeth II Med Ctr, Dept Microbiol, Perth,Univ Western Australia Perth WA Australia 6009 tr, Dept Microbiol, Perth,
Titolo Testata:
CLINICAL AND EXPERIMENTAL ALLERGY
fascicolo: 10, volume: 28, anno: 1998,
pagine: 1273 - 1285
SICI:
0954-7894(199810)28:10<1273:CLOPAN>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSEPITHELIAL ELECTRICAL-RESISTANCE; BRONCHIAL-MUCOSA; HAEMOPHILUS-INFLUENZAE; IN-VITRO; ALLERGEN; ASTHMA; EXPRESSION; HISTAMINE; CLEARANCE; CD23;
Keywords:
proteinase allergen; house dust mite; Der p 1; Der p 3; Der p 6; Der p 9; epithelial cohesion; epithelial permeability; airway microinjury; tight junctions;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Robinson, C St Georges Hosp, Sch Med, Dept Pharmacol & Clin Pharmacol, Cranmer Terrace, St Georges Hosp Cranmer Terrace London England SW17 0RE race,
Citazione:
H.L. Winton et al., "Cell lines of pulmonary and non-pulmonary origin as tools to study the effects of house dust mite proteinases on the regulation of epithelial permeability", CLIN EXP AL, 28(10), 1998, pp. 1273-1285

Abstract

Background Allergenic and non-allergenic proteinases from house dust mites(HDM) cause loss of adhesion between airway epithelial cells that may result in a loss of functional cohesion between the cells and thus assist in allergen presentation. Improved cellular assay systems are needed to ascertain the mechanisms involved. Objectives To survey a series of epithelial cell lines (Calu-3, 16HBE14o(-), NCI-H292 and A549 from human airways, and MDCK from dog kidney) and establish their utility for studies of the effects of HDM proteinases from D. pteronyssinus on epithelial permeability, To develop an improved method for measuring changes in epithelial permeability induced by HDM proteinases andother provocants. Methods The permeability of epithelial monolayer cultures to mannitol was calculated from measurements of clearance using a technique that permits mathematical estimation and reduction of non-cellular diffusional constraints. Permeability was studied under control conditions and after perturbation of monolayers with HDM proteinases (separated into serine- and cysteine-proteinase classes) or chelation of extracellular Ca2+. Fluorescent antibody staining was used to investigate whether the cells expressed tight junctions(staining of ZO-1), desmosomes (staining of desmoplakin) and zonulae adherentes (staining of E-cadherin). Results The Calu-3 line was identified as an airway cell line that expressed functional light junctions, desmosomes and zonulae adherentes. Calu-3 monolayers exhibited a low clearance and permeability to mannitol, similar tothat seen in the extensively characterized MDCK cell line. Clearance and permeability were significantly increased by treatment with either HDM proteinase fraction or by calcium chelation. 16HBE14o(-) cells also had a low permeability to mannitol under control conditions and expressed a similar repertoire of functional proteins from major intercellular junctions. In contrast, NCI-H292 and A549 cell lines were functionally deficient in tight junctions, although they did express desmosomes and zonulae adherentes to a greater extent. Epithelial permeability was found to be a more appropriate andsensitive index of epithelial perturbation than was tracer clearance. Conclusion These results suggest that the Calu-3 and 16HBE14o(-) cell lines are useful tools in studying the mechanism of HDM proteinases on airway epithelial cell function. HDM proteinases of both cysteine and serine mechanistic classes were found to perturb epithelial adhesion and function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:27:20