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Titolo:
Multiple effects of transfection with interleukin 2 and/or interferon gamma on the behavior of mouse T lymphoma cells
Autore:
Geldhof, AB; Van Ginderachter, J; Vandersteen, P; Raes, G; De Baetselier, P;
Indirizzi:
Freeb,niv Brussels, Flanders Interuniv Inst Biotechnol, Cellular Immunol La Free Univ Brussels Rhode St Genese Belgium B-1640 l, Cellular Immunol La
Titolo Testata:
CLINICAL & EXPERIMENTAL METASTASIS
fascicolo: 5, volume: 16, anno: 1998,
pagine: 447 - 459
SICI:
0262-0898(199807)16:5<447:MEOTWI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; COLONY-STIMULATING FACTOR; ANTITUMOR IMMUNITY; GENE-TRANSFER; FACTOR-ALPHA; IN-VIVO; ANTIGENS; TUMORIGENICITY; EXPRESSION; INVIVO;
Keywords:
IFN-gamma; IL-2; metastasis; T lymphoma cell; transfection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Geldhof, AB Freeb,niv Brussels, Flanders Interuniv Inst Biotechnol, Cellular Immunol La Free Univ Brussels Paardenstr 65 Rhode St Genese Belgium B-1640
Citazione:
A.B. Geldhof et al., "Multiple effects of transfection with interleukin 2 and/or interferon gamma on the behavior of mouse T lymphoma cells", CLIN EXP M, 16(5), 1998, pp. 447-459

Abstract

We have previously reported that transfection of mouse interferon gamma (IFN-gamma) in H-2K(k)-positive BW variants (BW-Sp3) abolishes tumorigenicityand reduces metastatic capacity. To further increase the immunogenicity ofBW-Sp3 cells, the gene for human interleukin 2 (huIL-2) was transfected inthese cancer cells, Single BW-Sp3(huIL-2) and double BW-Sp3(huIL-2+IFN-gamma) transfectants were generated and their behavior was investigated as compared to parental and IFN-gamma-transfected BW-Sp3, Although expression of huIL-2 was equally effective as IFN-gamma in preventing tumor formation andreducing experimental metastasis, it did not confer protection to spontaneous metastases and even reversed the anti-metastatic activity of IFN-gamma,Inoculation of the BW variants in immunocompromised mice revealed that expression of IL-2 activates both T cells and aspecific immune effecters. However, in immunocompromised mice a clear pro-metastatic effect of IL-2 was recorded, Analysis of membrane antigens on the different variants showed a selective effect of huIL-2 on the expression of two surface antigens, i.e. L-selectin and metastatic T cell hybridoma antigen (MTH), which may contribute to metastasis, Hence upon expression of huIL-2 in T lymphoma variants, tumor outcome will depend on the balanced effects of the transfected cytokines on the immune response and the redirected effect on tumor progression. (C) 1998 Kluwer Academic Publishers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:23:49