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Titolo:
Integrin alpha 5 beta 1: a potent inhibitor of experimental lung metastasis
Autore:
Schirner, M; Herzberg, F; Schmidt, R; Streit, M; Schoning, M; Hummel, M; Kaufmann, C; Thiel, E; Kreuser, ED;
Indirizzi:
Schering AG, Expt Oncol, Res Labs, D-13342 Berlin, Germany Schering AG Berlin Germany D-13342 ol, Res Labs, D-13342 Berlin, Germany Freermany Berlin, Med Ctr Benjamin Franklin, Dept Pathol, D-1000 Berlin, Ge Free Univ Berlin Berlin Germany D-1000 n, Dept Pathol, D-1000 Berlin, Ge
Titolo Testata:
CLINICAL & EXPERIMENTAL METASTASIS
fascicolo: 5, volume: 16, anno: 1998,
pagine: 427 - 435
SICI:
0262-0898(199807)16:5<427:IA5B1A>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
MELANOMA-CELLS; NUDE-MICE; FIBRONECTIN; RECEPTOR; EXPRESSION; TUMORIGENICITY; PHENOTYPE; GROWTH;
Keywords:
extravasation; fibronectin; integrin alpha 5 beta 1; lung colonization; metastasis; mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Schirner, M Schering AG, Expt Oncol, Res Labs, D-13342 Berlin, Germany Schering AG Berlin Germany D-13342 , D-13342 Berlin, Germany
Citazione:
M. Schirner et al., "Integrin alpha 5 beta 1: a potent inhibitor of experimental lung metastasis", CLIN EXP M, 16(5), 1998, pp. 427-435

Abstract

The integrin alpha 5 beta 1 seems to be the most relevant receptor of tumor cells for binding to fibronectin, Although numerous studies suggest a role of tumor cell fibronectin interaction in tumor metastasis, differential integrin expression on tumor cells has, however, not been correlated with metastatic capabilities, We addressed this question by transfection of the integrin alpha 5 beta 1 cDNA into HT-29 human colon carcinoma cells which ledto de novo expression of functional integrin alpha 5 beta 1. Similar to other reports, expression of the integrin alpha 5 beta 1 in HT-29 tumor cellsexerted an inhibitory action on sell proliferation as indicated in our study by formation of fewer colonies in soft agar, The tumor growth inhibitoryproperty of the integrin alpha 5 beta 1 was also shown by reduction of subcutaneous xenograft growth in nude mice to approximately 50% of that of control transfectants, For the first time, we found that several clones of integrin alpha 5 subunit transfectants displayed dramatically reduced formation of lung colonies and cutaneous metastasis after intravenous injection into nude mice, While most animals inoculated with control transfectant cells formed macroscopically visible lung colonies ranging from 12.6 +/- 2.6 to 22.0 +/- 6.6 (mean colony number +/- SEM), mice inoculated with HT-29 cell clones expressing the integrin alpha 5 beta 1 were almost completely free oflung colonies (ranging from 0.0 +/- 0 to 0.2 +/- 0.1). Our results imply that integrin alpha 5 beta 1 expression inhibits circulating tumor cells in pursuing late steps of the metastatic process as represented by the artificial metastasis (lung colonisation) model, (C) 1998 Kluwer Academic Publishers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 18:07:27