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Titolo:
Expression of the steroidogenic acute regulatory protein and luteinizing hormone receptor and their regulation by tumor necrosis factor alpha in rat corpora lutea
Autore:
Chen, YJ; Feng, Q; Liu, YX;
Indirizzi:
Chineseplesd Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100080, Peo Chinese Acad Sci Beijing Peoples R China 100080 iol, Beijing 100080, Peo
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 2, volume: 60, anno: 1999,
pagine: 419 - 427
SICI:
0006-3363(199902)60:2<419:EOTSAR>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBONUCLEIC-ACID EXPRESSION; CORPUS-LUTEUM; GRANULOSA-CELLS; STAR GENE; IN-VIVO; PROLACTIN; OVARY; PORCINE; PROSTAGLANDIN-F2-ALPHA; PROGESTERONE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Liu, YX Chineseplesd Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100080, Peo Chinese Acad Sci Beijing Peoples R China 100080 jing 100080, Peo
Citazione:
Y.J. Chen et al., "Expression of the steroidogenic acute regulatory protein and luteinizing hormone receptor and their regulation by tumor necrosis factor alpha in rat corpora lutea", BIOL REPROD, 60(2), 1999, pp. 419-427

Abstract

Expression of both mRNA and protein of the steroidogenic acute regulatory protein (StAR), in correlation with progesterone (P) production and LH receptor (LHR) mRNA expression, was studied in the corpora lutea (CL) of gonadotropin-induced-pseudopregnant and pregnant rats at various stages of CL development. Immature female rats, 21-22 days old, were injected s.c. with 20 IU eCG to stimulate follicle growth and then with 20 IU hCG 48 h later to induce ovulation. The ovaries were removed at various stages of CL development; either CL were isolated and snap frozen for total RNA analysis, or whole ovaries were fixed in Bouin's fluid for paraffin sectioning. The results of in situ hybridization, immunohistochemistry, and Northern blotting showed that the increase in StAR mRNA and protein expression was well correlatedwith the increase in serum P concentration. StAR expression was restrictedto the luteal cells or theca cells in antral follicles. Both StAR mRNA andprotein in the CL of pseudopregnant rats increased steadily on Day 1 and Day 4, reached highest levels on Day 4, and then dropped sharply on Day 8 when luteolysis takes place. LHR mRNA content was high on Day 1 but dropped significantly on Day 2. LHR mRNA increased to high levels on Day 4 and 8 andthen declined on Day 12. StAR mRNA and protein levels in the CL of pregnant rats were high during early luteal development (Day 2, 4), increased evenfurther on Day 9, and decreased on Day 13 when luteolysis takes place. It is therefore suggested that the expression of StAR coincides well with the capacity of P production in the CL and that StAR expression can be used as a functional "marker" of CL development. To study the possible effect of cytokines on StAR expression, pseudopregnant rats on Day 5 were injected s.c. with 10 IU hCC plus 20 mu g prolactin (PRL), with or without 500 IU tumor necrosis factor alpha (TNF alpha) 30 minlater. TNF alpha significantly inhibited hCG/PRL-induced StAR and LHR mRNAexpression at 1 and 3 h post-TNF alpha. It is suggested that the luteolytic effect of TNF alpha may be mediated by its direct inhibition on StAR expression or by an indirect decrease in LHR expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 07:20:56