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Titolo:
Effect of electric field on physical states of cell-associated water in germinating morning glory seeds observed by H-1-NMR
Autore:
Isobe, S; Ishida, N; Koizumi, M; Kano, H; Hazlewood, CF;
Indirizzi:
Natl Food Res Inst, Tsukuba, Ibaraki 305842, Japan Natl Food Res Inst Tsukuba Ibaraki Japan 305842 ba, Ibaraki 305842, Japan Natl Inst Agrobiol Resources, Tsukuba, Ibaraki 3058602, Japan Natl Inst Agrobiol Resources Tsukuba Ibaraki Japan 3058602 3058602, Japan Baylor Coll Med, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 ylor Coll Med, Houston, TX 77030 USA
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
fascicolo: 1, volume: 1426, anno: 1999,
pagine: 17 - 31
SICI:
0304-4165(19990104)1426:1<17:EOEFOP>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-MAGNETIC-RESONANCE; ARTEMIA CYSTS; INTRACELLULAR WATER; RELAXATION-TIMES; PULSED NMR; DIFFUSION; MUSCLE;
Keywords:
germination; physical state of water; electric field; NMR; morning glory (Pharbitis nil) seed;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Isobe, S Natl Food Res Inst, Tsukuba, Ibaraki 305842, Japan Natl Food Res Inst Tsukuba Ibaraki Japan 305842 ki 305842, Japan
Citazione:
S. Isobe et al., "Effect of electric field on physical states of cell-associated water in germinating morning glory seeds observed by H-1-NMR", BBA-GEN SUB, 1426(1), 1999, pp. 17-31

Abstract

Morning glory seeds in dry conditions (0.099 g H2O/dry wt.) were exposed to electric fields and germinated. The physical state of water in the germinating seeds of both control and exposed groups were examined using H-1-NMR spectroscopy and NMR microscopy. Three water fractions were observed which were characterized by different relaxation times (T-1) and chemical shifts. The average region containing long T-1 fractions was approximately 50 mu min diameter and consisted of half-permeable barriers. The maximum intracellular water transport rate was 2.3 x 10(-5) cm(2)/s. The treatment with electric field (500 kV/m for 60 min) increased the fraction with the shortest T-1 and decreased that with the longest T-1. Because the total water content in the treated seeds (3.4 g HzO/dry wt.) was similar to that in the untreated seeds (3.9 g HzO/dry wt.), the treated seeds held more water in a condition in restricted motion than the untreated seeds. It is thought that themembrane systems were affected by the electric polarization which led to an unusual accumulation of water and the hydration of stored macromolecules during the imbibition process. This set of events led to excessive swellingof stored macromolecules, resulting in the disruption of membrane systems and irregular organization of tissue structures. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 19:27:29