Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Overview of N- and O-linked oligosaccharide structures found in various yeast species
Autore:
Gemmill, TR; Trimble, RB;
Indirizzi:
New York State Dept Hlth, Wadsworth Ctr C547, Albany, NY 12201 USA New York State Dept Hlth Albany NY USA 12201 r C547, Albany, NY 12201 USA
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
fascicolo: 2, volume: 1426, anno: 1999,
pagine: 227 - 237
SICI:
0304-4165(19990106)1426:2<227:OONAOO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-WALL MANNAN; OUTER CHAIN ELONGATION; SCHIZOSACCHAROMYCES-POMBE; CANDIDA-ALBICANS; SACCHAROMYCES-CEREVISIAE; PATHOGENIC YEAST; METHYLOTROPHIC YEAST; MANNOSE RESIDUES; PICHIA-PASTORIS; PROTEIN COMPLEX;
Keywords:
yeast glycoprotein; N-glycan; O-glycan; oligosaccharide structure; NMR spectroscopy; secretion;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
93
Recensione:
Indirizzi per estratti:
Indirizzo: Trimble, RB New York State Dept Hlth, Wadsworth Ctr C547, POB 509, Albany,NY 12201 USA New York State Dept Hlth POB 509 Albany NY USA 12201 12201 USA
Citazione:
T.R. Gemmill e R.B. Trimble, "Overview of N- and O-linked oligosaccharide structures found in various yeast species", BBA-GEN SUB, 1426(2), 1999, pp. 227-237

Abstract

Yeast and most higher eukaryotes utilize an evolutionarily conserved N-linked oligosaccharide biosynthetic pathway that involves the formation of a Glc(3)Man(9)GlcNAc(2)-PP-dolichol lipid-linked precursor, the glycan portionof which is cotranslationally transferred in the endoplasmic reticulum (ER) to suitable Asn residues on nascent polypeptides. Subsequently, ER processing glycohydrolases remove the three glucoses and, with the exception of Schizosaccharomyces pombe, a single, specific mannose residue. Processing sugar transferases in the Golgi lead to the formation of core-sized structures (Hex(<15)GlcNa(2)) as well as cores with an extended poly-alpha 1,6-Man 'backbone' that is derivatized with various carbohydrate side chains in a species-specific manner (Hex(50-200)GlnNAc(2)). In some cases these are shortalpha 1,2-linked Man chains with (Saccharomyces cerevisiae) or without (Pichia pastoris) alpha 1,3-Man caps, while in other yeast (S. pombe), the side chains are alpha 1,2-linked Gal, some of which are capped with beta-1,3-linked pyruvylated Gal residues. Charged groups are also found in S. cerevisiae and P. pastoris N-glycans in the form of mannose phosphate diesters. Some pathogenic yeast (Candida albicans) add poly-beta 1,2-Man extension through a phosphate diester to their N-glycans, which appears involved in virulence. O-Linked glycan synthesis in yeast, unlike in animal cells where it is initiated in the Golgi using nucleotide sugars, begins in the ER by addition of a single mannose from Man-P-dolichol to selected Ser/Thr residues innewly made proteins. Once transported to the Golgi, sugar transferases addone (C. albicans) or more (P. pastoris) alpha 1,2-linked mannose that may be capped with one or two alpha 1,3-linked mannoses (S. cerevisiae). S. pombe is somewhat unique in that it synthesizes a family of mixed O-glycans with additional alpha 1,2-linked Man and alpha 1,2- and 1,3-linked Gal residues. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 14:55:38