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Titolo:
AFM, a tool for single-molecule experiments
Autore:
Ludwig, M; Rief, M; Schmidt, L; Li, H; Oesterhelt, F; Gautel, M; Gaub, HE;
Indirizzi:
Univ Munich, Lehrstuhl Angew Phys, D-80799 Munich, Germany Univ Munich Munich Germany D-80799 l Angew Phys, D-80799 Munich, Germany Jilininaiv, Key Lab Supramol Struct & Spect, Changchun 130023, Peoples R Ch Jilin Univ Changchun Peoples R China 130023 angchun 130023, Peoples R Ch European Mol Biol Lab, Biol Struct Div, D-69012 Heidelberg, Germany European Mol Biol Lab Heidelberg Germany D-69012 012 Heidelberg, Germany
Titolo Testata:
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
fascicolo: 2, volume: 68, anno: 1999,
pagine: 173 - 176
SICI:
0947-8396(199902)68:2<173:AATFSE>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
ATOMIC-FORCE MICROSCOPE; IMMUNOGLOBULIN DOMAINS; TITIN; RECOGNITION; ELASTICITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
--discip_EC--
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Ludwig, M Univ Munich, Lehrstuhl Angew Phys, Amalienstr 54, D-80799 Munich, Germany Univ Munich Amalienstr 54 Munich Germany D-80799 unich, Germany
Citazione:
M. Ludwig et al., "AFM, a tool for single-molecule experiments", APPL PHYS A, 68(2), 1999, pp. 173-176

Abstract

Force versus elongation curves measured with AFM-related techniques reveala detailed insight into material properties at the molecular level. Here an overview on different implementations of this technique is given. The lateral distribution of a receptor on a sample surface was determined by mapping the adhesion force with a corresponding ligand immobilized at the tip, Adextran pattern on a gold surface was measured by mapping the rupture length of the polymer. Stretching single dextran molecules revealed a structural transition in the polysaccharide backbone at forces of 750 pN. The unfolding of individual domains in the modular protein titin was observed and information about the rate dependence of the unfolding forces was obtained.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 22:36:49