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Titolo:
Microvascular responses to hemodilution with Hb vesicles as red blood cellsubstitutes: influence of O-2 affinity
Autore:
Sakai, H; Tsai, AG; Rohlfs, RJ; Hara, H; Takeoka, S; Tsuchida, E; Intaglietta, M;
Indirizzi:
Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 ioengn, La Jolla, CA 92093 USA Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 pt Med, La Jolla, CA 92093 USA Wasedaanniv, Dept Polymer Chem, Adv Res Inst Sci & Engn, Tokyo 1698555, Jap Waseda Univ Tokyo Japan 1698555 Res Inst Sci & Engn, Tokyo 1698555, Jap
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 2, volume: 45, anno: 1999,
pagine: H553 - H562
SICI:
0363-6135(199902)45:2<H553:MRTHWH>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CROSS-LINKED HEMOGLOBIN; DISSOCIATION CURVE; OXYGEN-AFFINITY; CARDIAC-OUTPUT; NITRIC-OXIDE; FLOW; MICROCIRCULATION; TRANSPORT; EXCHANGE; RATS;
Keywords:
oxygen carrier; microcirculation; liposome; oxygen dissociation curve; autoregulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Intaglietta, M Univ Calif San Diego, Dept Bioengn, 9500 Gilman Dr, La Jolla, CA 92093 USA Univ Calif San Diego 9500 Gilman Dr La Jolla CA USA 92093 A
Citazione:
H. Sakai et al., "Microvascular responses to hemodilution with Hb vesicles as red blood cellsubstitutes: influence of O-2 affinity", AM J P-HEAR, 45(2), 1999, pp. H553-H562

Abstract

Phospholipid vesicles encapsulating purified hemoglobin (HbV) were developed to provide O-2-carrying capacity to plasma expanders. Microvascular perfusion was determined for HbV with different O-2 affinity (P-50 = 9, 16, and30 mmHg) prepared by coencapsulating pyridoxal 5'-phosphate (PLP) at the molar ratios of[PLP]/[Hb] = 0, 0.5, and 3, respectively (cf. hamster blood, P-50: 28 mmHg), and suspended in 8 g/dl human serum albumin (HSA). Eighty percent of the red blood cell (RBC) mass of conscious Syrian golden hamstersfitted with dorsal skinfold windows was substituted with either of the HbV-HSA suspensions, washed hamster RBC suspended in HSA(RBC-HSA), and HSA alone. All three HbV-HSA groups and RBC-HSA groups showed stable blood pressure and heart rate, which could not be sustained with HSA alone. Only the HbV(P-50 = 9)-HSA group showed an increase in arterial O-2 tension (89.8 +/- 14.7 mmHg, baseline 58.4 +/- 4.0 mmHg) because of hyperventilation, and microvascular perfusion was decreased, indicating that facilitated O-2 unloading of HbV by decreasing the O-2 affinity (increasing P-50) with PLP as an allosteric effector is important. Microvascular perfusion and microvascular and interstitial O-2 tensions in the HbV (P-50 = 16 and 30)-HSA groups weresignificantly higher than those in the HSA group. The O-2 release rate from the HbV was 18-32 s(-1) vs. 4.4 s(-1) for RBC. Functional capillary density was improved from 17 to 41% on average by decreasing P-50 from 30 to 16 mmHg, which appears to be an optimal value for the P-50 in this system.

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Documento generato il 29/03/20 alle ore 15:24:11