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Titolo:
High susceptibility of Mongolian gerbil (Meriones unguiculatus) to Borna disease virus
Autore:
Nakamura, Y; Nakaya, T; Hagiwara, K; Momiyama, N; Kagawa, Y; Taniyama, H; Ishihara, C; Sata, T; Kurata, T; Ikuta, K;
Indirizzi:
Hokkaido0815,, Inst Immunol Sci, Sect Serol, Kita Ku, Sapporo, Hokkaido 060 Hokkaido Univ Sapporo Hokkaido Japan 0600815 ta Ku, Sapporo, Hokkaido 060 Rakuno Gakuen Univ, Sch Vet Med, Ebetsu, Hokkaido 0698501, Japan Rakuno Gakuen Univ Ebetsu Hokkaido Japan 0698501 Hokkaido 0698501, Japan Natl Inst Infect Dis, Dept Pathol, Shinjuku Ku, Tokyo 1628640, Japan Natl Inst Infect Dis Tokyo Japan 1628640 injuku Ku, Tokyo 1628640, Japan
Titolo Testata:
VACCINE
fascicolo: 5, volume: 17, anno: 1999,
pagine: 480 - 489
SICI:
0264-410X(19990205)17:5<480:HSOMG(>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERIPHERAL-BLOOD MONONUCLEAR; NITRIC-OXIDE SYNTHASE; PSYCHIATRIC-PATIENTS; NERVOUS-SYSTEM; INFECTED-RATS; CELLS; RNA; BRAIN; PERSISTENT; PREVALENCE;
Keywords:
BDV; animal model; gerbil; inflammation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Ikuta, K Hokkaido0815,, Inst Immunol Sci, Sect Serol, Kita Ku, Sapporo, Hokkaido 060 Hokkaido Univ Sapporo Hokkaido Japan 0600815 pporo, Hokkaido 060
Citazione:
Y. Nakamura et al., "High susceptibility of Mongolian gerbil (Meriones unguiculatus) to Borna disease virus", VACCINE, 17(5), 1999, pp. 480-489

Abstract

Borna disease virus (BDV) is a neurotropic enveloped virus with a nonsegmented, single-, negative-stranded RNA genome. This virus induced encephalitis in experimentally infected adult rats, but in newborn rats BDV established a persistent, tolerant infection with no apparent clinical signs. Here, we report evidence that newborn Mongolian gerbils (Meriones umguiculatus) are more susceptible to experimental intracranial inoculation of horse-derived BDV in persistently infected MDCK cells, compared with similar inoculation in newborn rats. All inoculated newborn gerbils, but not rats, died 30 days after infection. Reverse transcriptase-polymerase chain reaction amplified BDV-specific sequences in several regions including the brain. Histopathological analysis revealed apparent inflammatory reactions in the brains ofinoculated gerbils but not rats, although similar levels of BDV RNA were detected in both gerbil and rat brains. BDV-specific antigen and RNA were identified predominantly in neurons in the brains by immunohistochemistry with antibodies to BDV and in situ hybridization with BDV-specific riboprobes,respectively BDV in the gerbil brain was easily rescued by co-cultivation of the brain homogenate with human oligodendroglioma cells. Thus, gerbils seem to be a useful animal model for studying BDV-induced pathogenesis in the brain. (C) 1999 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 01:32:49