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Titolo:
Matrix metalloproteinase-2 in a murine model of infantile-type polycystic kidney disease
Autore:
Rankin, CA; Itoh, Y; Tian, CQ; Ziemer, DM; Calvet, JP; Gattone, VH;
Indirizzi:
Univ Kansas, Med Ctr, Dept Anat & Cell Biol, Kansas City, KS 66160 USA Univ Kansas Kansas City KS USA 66160 Cell Biol, Kansas City, KS 66160 USA Univ Kansas, Med Ctr, Dept Biochem & Mol Biol, Kansas City, KS 66160 USA Univ Kansas Kansas City KS USA 66160 Mol Biol, Kansas City, KS 66160 USA
Titolo Testata:
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
fascicolo: 2, volume: 10, anno: 1999,
pagine: 210 - 217
SICI:
1046-6673(199902)10:2<210:MMIAMM>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHENOL-CHLOROFORM EXTRACTION; SINGLE-STEP METHOD; HAN-SPRD RAT; EXTRACELLULAR-MATRIX; IV COLLAGENASE; RNA ISOLATION; EXPRESSION; GELATINASE; FIBROSIS; ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Gattone, VH Univ Kansas, Med Ctr, Dept Anat & Cell Biol, Kansas City, KS 66160 USA Univ Kansas Kansas City KS USA 66160 ansas City, KS 66160 USA
Citazione:
C.A. Rankin et al., "Matrix metalloproteinase-2 in a murine model of infantile-type polycystic kidney disease", J AM S NEPH, 10(2), 1999, pp. 210-217

Abstract

It was previously found that elevated levels of matrix metalloproteinase (MMP)-2 (gelatinase A) and -9 (gelatinase B) were synthesized and secreted into the medium by cultured kidney tubules derived from cystic C57BL/6J-cpk mice. To determine whether increased synthesis and secretion occur in vivo in this mouse model of polycystic kidney disease, kidney protein extracts, mRNA, and tissue sections were compared for expression and activity of MMP-2 and -9. Although both MMP were detected in tissue extracts, the differences in expression levels and activity in normal and cystic kidneys were far greater for MMP-2. High levels of MMP-2 seemed to result from increased expression by the cystic kidneys predominantly in the second and third postnatal weeks (a time when the kidneys are undergoing rapid cystic enlargement). Much of the increased MMP was present in the inactive zymogen form, although active enzyme was readily detected by sodium dodecyl sulfate-polyacrylamide gel zymography and in situ zymography. MMP-2 was abnormally localized to the interstitium and to foci between cysts, suggesting that MMP-2 may regulate collagen accumulation at those sites, thus allowing cyst enlargement and limiting the severity of interstitial fibrosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 16:14:58