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Titolo:
Triploid induction in Pacific abalone Haliotis discus hannai Ino by 6-dimethylaminopurine and the performance of triploid juveniles
Autore:
Zhang, GF; Wang, ZC; Chang, YQ; Song, J; Ding, J; Wang, YP; Wang, RB;
Indirizzi:
Dalianhinaheries Univ, Key Lab Mariculture Ecol, Dalian 116023, Peoples R C Dalian Fisheries Univ Dalian Peoples R China 116023 116023, Peoples R C Dalian Pacific Sea Food Ltd, Dalian 116041, Peoples R China Dalian PacificSea Food Ltd Dalian Peoples R China 116041 eoples R China
Titolo Testata:
JOURNAL OF SHELLFISH RESEARCH
fascicolo: 3, volume: 17, anno: 1998,
pagine: 783 - 788
SICI:
0730-8000(199812)17:3<783:TIIPAH>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRASSOSTREA-GIGAS; CYTOCHALASIN-B; MOUSE OOCYTE; OYSTER; TEMPERATURE; MATURATION; PUROMYCIN; GROWTH; 6-DMAP;
Keywords:
abalone; triploid; 6-dimethylaminopurine (6-DMAP);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Zhang, GF Dalianhinaheries Univ, Key Lab Mariculture Ecol, Dalian 116023, Peoples R C Dalian Fisheries Univ Dalian Peoples R China 116023 eoples R C
Citazione:
G.F. Zhang et al., "Triploid induction in Pacific abalone Haliotis discus hannai Ino by 6-dimethylaminopurine and the performance of triploid juveniles", J SHELLFISH, 17(3), 1998, pp. 783-788

Abstract

Pressure shock, thermal shock, and cytochalasin B treatment have been the main treatments used in inducing triploid gastropod. In this study 6-dimethylaminopurine (6-DMAP) was attempted to induce triploid in Pacific abalone,Haliotis discus hannai Ino, by inhibiting formations of either polar body 1 (pb1) or polar body 2 (pb2). Although the highest triploid (73.5%) was obtained by blocking pb2 for 15 min with a 6-DMAP concentration as high as 300 mu M (the recommended dose for triploid induction in bivalve), no veligers could survive. At the ranges of 75-150 mu M 6-DMAP, blacking pb2 for 20 min resulted in higher abnormalities than blocking pb2 for 15 min. The relative survival rate was higher and the abnormality was lower in the pb2 groupthan those in the pbl group. No significant difference in triploid induction (p > 0.05) was found both between 15- and 20-min treatments and between treatments targeting pb1 and pb2 at 75-150 mu M 6-DMAP. At 6-DMAP concentrations of 75, 100, 125, and 150 mu M, in groups that blocked the ph 2 formation, the triploid yields (number of triploid larvae/fertilized eggs) were 30.0, 46.0, 47.0, and 54.0% and the relative survivorships at the trochophore stage were 96.8, 95.3, 94.8, and 90.5% for 15-min treatments, respectively. The triploid yields were 30.1, 49.0, 51.2, and 56.0% and the relative survivorships at the trochophore stage were 95.8, 90.2, 88.5, and 82.6% for 20-min treatments, respectively; in groups that blocked pbl for 15 min, the triploid yields were 30.4, 43.0, 46.6, and 51.0% and the relative survivorships at the trochophore stage were 70.6, 68.4, 68.0, and 61.8%, respectively. The shell dimensions of triploids and controls were measured at 4 mo postfertilization. In the large-size group (1.2-1.3 cm), differences between 3n and 2n groups in both dimensions and weight were significant (p < 0.01), whereas in the small-size group (0.7-0.8 cm), no significant differences were found in dimensions (p > 0.05), but they were found in total weight (p <0.01). Overall, the optimal treatment criteria for triploid production in Pacific abalone with 6-DMAP appear to be 125-150 mu M for 15-20 min at 500-600/mL zygote density and 23.0 degrees C.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 05:14:23