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Titolo:
Plastid gene expression during amyloplast formation in cultured tobacco cells
Autore:
Sakai, A; Miyazawa, Y; Suzuki, T; Sasaki, N; Kawano, S; Kuroiwa, T;
Indirizzi:
Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Sci, Dept Biol Sci, Tokyo 1130033, Japan
Titolo Testata:
JOURNAL OF PLANT PHYSIOLOGY
fascicolo: 1, volume: 154, anno: 1999,
pagine: 71 - 78
SICI:
0176-1617(199901)154:1<71:PGEDAF>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
NICOTIANA-TABACUM-L; SINAPIS-ALBA L; TRANSCRIPTIONAL REGULATION; CHLOROPLAST GENOME; NUCLEI NUCLEOIDS; DNA; PROTEINS; NUMBER; MULTIPLICATION; IDENTIFICATION;
Keywords:
Nicotiana tabacum; amyloplast; plastid-nuclei (nucleoids); transcription;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Sakai, A Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 t Biol Sci, Tokyo 1130033, Japan
Citazione:
A. Sakai et al., "Plastid gene expression during amyloplast formation in cultured tobacco cells", J PLANT PHY, 154(1), 1999, pp. 71-78

Abstract

We analyzed changes in plastid gene expression during amyloplast formationin cultured tobacco (Nicotiana tabacum L.) cells. When cultured tobacco cells (line BY-2) in the stationary phase were transferred to auxin-depleted culture medium that contained cytokinin, leucoplast-like plastids in the BY-2 cells were converted td amyloplasts within two days. The cell number, number of plastids per cell, and the relative amount of plastid DNA per cell remained nearly constant throughout this process. During amyloplast formation, transcripts for most plastid genes increased only transiently, and stopped increasing during the late phase of amyloplast development, while transcript levels for psbA (a gene for D1 protein of photosystem II) and psaA/B (genes for P700 apoprotein A1/A2 of photosystem I) continued to increase. Assay of transcription in vitro using isolated plastid-nuclei (nucleoids) showed that the transcriptional activities of most plastid genes decreased dramatically during this process, whereas the transcription of psbA and psaA/B continued at a considerable rate, showing a correlation between transcription and transcript accumulation. However, the fact that increases in transcript abundance were observed for all the plastid genes investigated here in spite of the marked decrease in their transcriptional activity suggests that the degradation of transcripts may also be inactive in the amyloplasts of BY-2 cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 01:08:03