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Titolo:
Protein kinase C modulation of recombinant NMDA receptor currents: Roles for the C-terminal C1 exon and calcium ions
Autore:
Logan, SM; Rivera, FE; Leonard, JP;
Indirizzi:
Univ Illinois, Dept Biol Sci, Chicago, IL 60607 USA Univ Illinois ChicagoIL USA 60607 , Dept Biol Sci, Chicago, IL 60607 USA Cayey Univ Coll, Dept Biol, Cayey, PR 00736 USA Cayey Univ Coll Cayey PR USA 00736 v Coll, Dept Biol, Cayey, PR 00736 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 3, volume: 19, anno: 1999,
pagine: 974 - 986
SICI:
0270-6474(19990201)19:3<974:PKCMOR>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXCITATORY POSTSYNAPTIC CURRENTS; DEPENDENT CHLORIDE CURRENT; D-ASPARTATE RECEPTORS; XENOPUS OOCYTES; RAT-BRAIN; SPLICE VARIANTS; MESSENGER-RNAS; NR1 SUBUNIT; SYNAPTIC PLASTICITY; PHOSPHORYLATION;
Keywords:
NR1; NR2; NMDAR; alternative splicing; variants; Xenopus oocytes; barium; phorbol ester; homomer; heteromer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Logan, SM Univ Illinois, Dept Biol Sci, Chicago, IL 60607 USA Univ Illinois Chicago IL USA 60607 l Sci, Chicago, IL 60607 USA
Citazione:
S.M. Logan et al., "Protein kinase C modulation of recombinant NMDA receptor currents: Roles for the C-terminal C1 exon and calcium ions", J NEUROSC, 19(3), 1999, pp. 974-986

Abstract

Protein kinase C (PKC) positively modulates NMDA receptor (NMDAR) currents. in contrast to previous reports, this study determines the importance of individual exons in the mechanism underlying the potentiation process by examining the complete set of eight naturally occurring splice variants expressed in Xenopus oocytes both as homomers and as heteromeric NR1/NR2A or NR1/NR2B complexes. After PKC stimulation, homomeric currents demonstrated a high revel of potentiation (similar to 500% of untreated baseline currents) that reduced to a lower level (similar to 300% of baseline) in variants containing the first C-terminal exon (C1). An ANOVA showed that only Cf and noother exon or interaction of exons determined the degree of NMDAR current modulation by PKC. When recordings were performed in solutions in which barium replaces calcium, only the lower form of potentiation was observed, regardless of the splice variant exon composition. This suggested an importantrole for calcium in the PKC modulation of homomeric NMDA splice variant currents in which the C1 exon also participates. The effectiveness of the C1 exon to reduce the higher form of potentiation is modulated by heteromeric assemblies with NR2A heteromers yielding smaller levels of potentiation anda larger C1 exon effect compared with NR2B heteromers. The heteromers demonstrated the higher form of potentiation even in the absence of calcium. Furthermore, calcium had different effects in the potentiation of the heteromers depending on the NR2 subunit. This study refines the region of the NR1 subunit involved in a modulation crucial to the function of NMDA receptors and provides evidence that the NR2A and NR2B subunits realize this modulation differentially.

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Documento generato il 07/08/20 alle ore 07:11:01