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Titolo:
Tissue-specific levels and cellular distribution of epidermal growth factor receptors within control and neomycin-damaged neonatal rat organ of corti
Autore:
Zine, A; de Ribaupierre, F;
Indirizzi:
Univ Lausanne, Inst Physiol, CH-1005 Lausanne, Switzerland Univ Lausanne Lausanne Switzerland CH-1005 CH-1005 Lausanne, Switzerland
Titolo Testata:
JOURNAL OF NEUROBIOLOGY
fascicolo: 3, volume: 38, anno: 1999,
pagine: 313 - 322
SICI:
0022-3034(19990215)38:3<313:TLACDO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
EAR SENSORY EPITHELIA; MAMMALIAN INNER-EAR; ACOUSTIC TRAUMA; HAIR-CELLS; FACTOR-ALPHA; SIGNAL-TRANSDUCTION; CHICK COCHLEA; INTENSE SOUND; REGENERATION; PROLIFERATION;
Keywords:
auditory hair cells; ototoxicity; organotypic culture; epidermal growth factor receptor; EGF; immunohistochemistry; ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: de Ribaupierre, F Univ Lausanne, Inst Physiol, 7 Rue Bugnon, CH-1005 Lausanne, Switzerland Univ Lausanne 7 Rue Bugnon Lausanne Switzerland CH-1005
Citazione:
A. Zine e F. de Ribaupierre, "Tissue-specific levels and cellular distribution of epidermal growth factor receptors within control and neomycin-damaged neonatal rat organ of corti", J NEUROBIOL, 38(3), 1999, pp. 313-322

Abstract

Epidermal growth factor receptor (EGFR) levels were assayed by an enzyme-linked immunosorbent assay (ELISA) in microdissected organ of Corti (OC) from neonatal rats directly after isolation and after 3 days in culture with and without neomycin treatment. In addition, the cellular distribution of the EGFR in the OC was determined by immunohistochemistry, The in vitro levelof EGFR determined by ELISA assays doubled after neomycin damage to OC, suggesting that EGFR is subject to up-regulation following this treatment. Immunohistochemistry of both in vivo and in vitro controls indicates that EGFR is predominantly localized in the stereociliary bundles of the hair cells; supporting cells and the apical junctions between the remaining Kolliker organ cells were also immunolabeled, In neomycin-treated cultures, sensory cells were degenerated, so no labeling could be seen. However, supporting and Kolliker organ cells continued to show labeling. In addition, nerve fibers in the region of the future osseous spiral lamina and projecting out toward the damaged sensory epithelium were immunostained, The up-regulation ofthe EGFR and its redistribution within the OC following neomycin damage support the earlier observation that growth factors that act through EGFR, such as EGF and transforming growth factor-a can induce neonatal mammalian auditory hair cell replacement under culture conditions, after aminoglycosidetreatment. (C) 1999 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:27:10