Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification of autoimmune T cells among in vivo expanded CD25(+) T cells in multiple sclerosis
Autore:
Illes, Z; Kondo, T; Yokoyama, K; Ohashi, T; Tabira, T; Yamamura, T;
Indirizzi:
Natling, Neurol & Psychiat, Natl Inst Neurosci, Dept Demyelinating Dis & Ag Natl Ctr Neurol & Psychiat Tokyo Japan 1870031 pt Demyelinating Dis & Ag
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 3, volume: 162, anno: 1999,
pagine: 1811 - 1817
SICI:
0022-1767(19990201)162:3<1811:IOATCA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYELIN BASIC-PROTEIN; PROTEOLIPID PROTEIN; CLONALITY ANALYSIS; PERIPHERAL-BLOOD; CLONOTYPES; PEPTIDE; DISEASE; LESIONS; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Yamamura, T Natling, Neurol & Psychiat, Natl Inst Neurosci, Dept Demyelinating Dis & Ag Natl Ctr Neurol & Psychiat 4-1-1 Ogawahigashi Tokyo Japan 1870031
Citazione:
Z. Illes et al., "Identification of autoimmune T cells among in vivo expanded CD25(+) T cells in multiple sclerosis", J IMMUNOL, 162(3), 1999, pp. 1811-1817

Abstract

Although clonal expansion of autoimmune T cells has been reported in multiple sclerosis (MS), very limited information is available on specificities,clonal size, or activation state of the expanded clones. Here we address the issue of clonal expansion by using a novel technique demonstrating clonotypes defined by single-strand conformation polymorphism of TCR beta-chain cDNAs, Examination of activated T cells (CD3(+)CD25(+)) isolated from the peripheral blood of MS revealed limited numbers (20-82) of expanded clones defined by single-strand conformation polymorphism clonotype. To estimate the Ag specificities of dominant clonotypes in the activated T cells, these samples were examined in parallel with Th1 T cell clones specific for myelinbasic protein or proteolipid protein (PLP) derived from the same patients. Analysis of two patients demonstrated that the dominant clonotypes would contain those specific for myelin basic protein or PLP. Although the majority of the clonotypes could be detected only transiently, a PLP95-116-specific clonotype was found to persist for over 1 yr. Thus, single-strand conformation polymorphism clonotype analysis allows us to monitor the kinetics of given T cell clones in vivo and could provide useful information for designing clonotype (Id)-specific manipulation of human diseases such as MS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/08/20 alle ore 22:52:56