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Titolo:
Roles of PilC and PilE proteins in pilus-mediated adherence of Neisseria gonorrhoeae and Neisseria meningitidis to human erythrocytes and endothelialand epithelial cells
Autore:
Scheuerpflug, I; Rudel, T; Ryll, R; Pandit, J; Meyer, TF;
Indirizzi:
Max Planck Inst Biol, Infekt Biol Abt, D-72076 Tubingen, Germany Max Planck Inst Biol Tubingen Germany D-72076 D-72076 Tubingen, Germany Max Planck Inst Infekt Biol, D-10117 Berlin, Germany Max Planck Inst Infekt Biol Berlin Germany D-10117 10117 Berlin, Germany
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 2, volume: 67, anno: 1999,
pagine: 834 - 843
SICI:
0019-9567(199902)67:2<834:ROPAPP>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIMBRIATED ESCHERICHIA-COLI; PATHOGENIC NEISSERIA; ANTIGENIC VARIATION; PHASE-VARIATION; TRANSFORMATION COMPETENCE; GONOCOCCAL PILI; ADHESION; EXPRESSION; VARIANTS; MUTANTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Meyer, TF Maxmanynck Inst Biol, Infekt Biol Abt, Spemannstr 34, D-72076 Tubingen, Ger Max Planck Inst Biol Spemannstr 34 Tubingen Germany D-72076 Ger
Citazione:
I. Scheuerpflug et al., "Roles of PilC and PilE proteins in pilus-mediated adherence of Neisseria gonorrhoeae and Neisseria meningitidis to human erythrocytes and endothelialand epithelial cells", INFEC IMMUN, 67(2), 1999, pp. 834-843

Abstract

Unlike other type 4 pill, the neisserial pill consist of at least two distinct proteins, the highly variable major subunit PilE forming the pilus fiber and the tip-associated adhesin PilC. PilC protein purified either from gonococci or from Escherichia coli interacted with different human epithelial cell lines, primary epithelial and endothelial cells. The binding of PilCprotein efficiently prevented the attachment of piliated Neisseria gonorrhoeae and Neissaria meningitidis to these cell types. Fluorescent beads coated with pill prepared from piliated wild-type N. gonorrhoeae also adhered to these cells, in contrast to beads coated with pill prepared from a piliated PilC-deficient mutant. In the latter case, the binding of fluorescent beads was restored after pretreatment of the pilus-loaded beads with purifiedPilC, Piliated wild-type N. gonorrhoeae, the piliated PilC-deficient mutant, and N. gonorrhoeae pill assembled in Pseudomonas aeruginosa agglutinatedhuman erythrocytes, while nonpiliated gonococci did not. Consistently, purified PilC did not agglutinate or bind to human erythrocytes, suggesting that N. gonorrhoeae PilE is responsible for pilus-mediated hemagglutination.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 22:09:58