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Titolo:
Cytotoxic action of Serratia marcescens hemolysin on human epithelial cells
Autore:
Hertle, R; Hilger, M; Weingardt-Kocher, S; Walev, I;
Indirizzi:
Univ Tubingen, D-72076 Tubingen, Germany Univ Tubingen Tubingen Germany D-72076 bingen, D-72076 Tubingen, Germany Univ Mainz, Inst Med Microbiol, D-55101 Mainz, Germany Univ Mainz Mainz Germany D-55101 t Med Microbiol, D-55101 Mainz, Germany
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 2, volume: 67, anno: 1999,
pagine: 817 - 825
SICI:
0019-9567(199902)67:2<817:CAOSMH>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTERIOPHAGE-T7 RNA-POLYMERASE; STAPHYLOCOCCAL ALPHA-TOXIN; ESCHERICHIA-COLI HEMOLYSIN; HELICOBACTER-PYLORI; BOUND HEMOLYSIN; ERYTHROCYTE-MEMBRANES; HUMAN-MONOCYTES; PROTEIN; PORE; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Hertle, R Univ Tubingen, Morgenstelle 28, D-72076 Tubingen, Germany Univ Tubingen Morgenstelle 28 Tubingen Germany D-72076 Germany
Citazione:
R. Hertle et al., "Cytotoxic action of Serratia marcescens hemolysin on human epithelial cells", INFEC IMMUN, 67(2), 1999, pp. 817-825

Abstract

Incubation of human epithelial cells with nanomolar concentrations of chromatographically purified Serratia marcescens hemolysin (ShlA) caused irreversible vacuolation and subsequent lysis of the cells. Vacuolation differed from vacuole formation by Helicobacter pylori VacA. Sublytic doses of ShlA led to a reversible depletion of intracellular ATP. Restoration to the initial ATP level was presumably due to the repair of the toxin damage and was inhibited by cycloheximide. Pores formed in epithelial cells and fibroblasts without disruption of the plasma membrane, and the pores appeared to be considerably smaller than those observed in artificial lipid membranes and in erythrocytes and did not allow the influx of propidium iodide or trypan blue. All cytotoxic effects induced by isolated recombinant ShlA were also obtained with exponentially growing S. marcescens cells. The previously suggested role of the hemolysin in the pathogenicity of S. marcescens is supported by these data.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 13:26:36