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Titolo:
Interferon regulatory factors regulate interleukin-1 beta-converting enzyme expression and apoptosis in vascular smooth muscle cells
Autore:
Horiuchi, M; Yamada, H; Akishita, M; Ito, M; Tamura, K; Dzau, VJ;
Indirizzi:
Harvardon,iv, Sch Med, Brigham & Womens Hosp, Cardiovasc Div,Dept Med, Bost Harvard Univ Boston MA USA 02115 mens Hosp, Cardiovasc Div,Dept Med, Bost
Titolo Testata:
HYPERTENSION
fascicolo: 1, volume: 33, anno: 1999,
pagine: 162 - 166
SICI:
0194-911X(199901)33:1<162:IRFRIB>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
II TYPE-2 RECEPTOR; TRANSCRIPTION FACTOR IRF-1; DEATH GENE CED-3; ANGIOTENSIN-II; ICE/CED-3 PROTEASE; NITRIC-OXIDE; INHIBITION; INDUCTION; ELEMENTS; IFN;
Keywords:
apoptosis; growth substances; interferons; interleukin-1; muscle, smooth, vascular;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Horiuchi, M Harvardrancis Sch Med, Brigham & Womens Hosp, Cardiovasc Div,Dept Med, 75 F Harvard Univ 75 Francis St,Thorn 13 Boston MA USA 02115 , 75 F
Citazione:
M. Horiuchi et al., "Interferon regulatory factors regulate interleukin-1 beta-converting enzyme expression and apoptosis in vascular smooth muscle cells", HYPERTENSIO, 33(1), 1999, pp. 162-166

Abstract

Apoptosis has been reported to play a pivotal role in vascular remodeling. However, cellular mechanisms of apoptosis in vascular smooth muscle cells (VSMCs) have not been well defined. In this study, we focused on interleukin-1 beta-converting enzyme (ICE), a key protease in the induction of apoptosis in lymphocytes and fibroblasts. We observed an increase in ICE mRNA expression in rat aortic VSMCs after serum depletion, with a peak at 12 hours and then a gradual decline. This was associated with DNA fragmentation, a hallmark of apoptosis and morphological changes of apoptosis. Treatment of these VSMCs with the ICE inhibitor N-(N-acetyl-tyrosinyl-valinyl-alaninyl)-3-amino-4-oxobutanoic acid (WAD-CHO) attenuated DNA fragmentation. The increased ICE mRNA expression was preceded by an increase in the mRNA expressionof interferon regulatory factor (IRF)-1, peaking at 6 hours after serum removal, and a rapid but transient decrease in IRF-2 mRNA expression, reaching a nadir at 3 hours after serum depletion. To demonstrate that these reciprocal changes in IRE-I and IRF-2 regulated ICE expression and induced apoptosis, we transfected antisense oligonucleotides for IRE-I and IRF-2 into VSMCs and examined ICE mRNA expression and apoptotic changes. IRF-1 antisensepretreatment attenuated the increase in ICE expression and reduced apoptotic changes, whereas IRF-2 antisense treatment increased ICE mRNA expressionand enhanced apoptotic changes. Taken together, our results suggest that serum growth factor depletion in VSMCs upregulates IRE-I and downregulates IRF-2, thereby increasing ICE expression and inducing apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:30:05