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Titolo:
Entry and integration of transplanted hepatocytes in rat liver plates occur by disruption of hepatic sinusoidal endothelium
Autore:
Gupta, S; Rajvanshi, P; Sokhi, R; Slehria, S; Yam, A; Kerr, A; Novikoff, PM;
Indirizzi:
Albert Einstein Coll Med, Marion Bessin Liver Res Ctr, Bronx, NY 10461 USAAlbert Einstein Coll Med Bronx NY USA 10461 Res Ctr, Bronx, NY 10461 USA Albert Einstein Coll Med, Ctr Canc Res, Bronx, NY 10461 USA Albert Einstein Coll Med Bronx NY USA 10461 Canc Res, Bronx, NY 10461 USA Albert Einstein Coll Med, Dept Med, Bronx, NY 10461 USA Albert Einstein Coll Med Bronx NY USA 10461 Dept Med, Bronx, NY 10461 USA Albert Einstein Coll Med, Dept Radiol, Bronx, NY 10461 USA Albert EinsteinColl Med Bronx NY USA 10461 t Radiol, Bronx, NY 10461 USA Albert Einstein Coll Med, Dept Pathol, Bronx, NY 10461 USA Albert EinsteinColl Med Bronx NY USA 10461 t Pathol, Bronx, NY 10461 USA
Titolo Testata:
HEPATOLOGY
fascicolo: 2, volume: 29, anno: 1999,
pagine: 509 - 519
SICI:
0270-9139(199902)29:2<509:EAIOTH>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-THERAPY; GROWTH-FACTOR; KUPFFER CELLS; IN-VIVO; HYPOXIA; EXPRESSION; REPOPULATION; ANGIOGENESIS; RELEVANCE; MECHANISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Gupta, S Albertrisnstein Coll Med, Marion Bessin Liver Res Ctr, Ullmann 625,1300 Mor Albert Einstein Coll Med Ullmann 625,1300 Morris Pk Ave Bronx NYUSA 10461
Citazione:
S. Gupta et al., "Entry and integration of transplanted hepatocytes in rat liver plates occur by disruption of hepatic sinusoidal endothelium", HEPATOLOGY, 29(2), 1999, pp. 509-519

Abstract

To establish the process by which transplanted cells integrate into the liver parenchyma, we used dipeptidyl peptidase IV-deficient F344 rats as hosts. On intrasplenic injection, transplanted hepatocytes immediately entered liver sinusoids, along with attenuation of portal vein radicles on angiography. However, a large fraction of transplanted cells (>70%) was rapidly cleared from portal spaces by phagocyte/macrophage responses. On the other hand, transplanted hepatocytes entering the hepatic sinusoids showed superior survival. These cells translocated from sinusoids into liver plates between16 and 20 hours after transplantation, during which electron microscopy showed disruption of the sinusoidal endothelium. Interestingly, production ofvascular endothelial growth factor was observed in hepatocytes before endothelial disruptions. Portal hypertension and angiographic changes resultingfrom cell transplantation resolved promptly. Integration of transplanted hepatocytes in the liver parenchyma required cell membrane regenesis, with hybrid gap junctions and bile canaliculi forming over 3 to 7 days after celltransplantation. We propose that strategies to deposit cells into distal hepatic sinusoids, to disrupt sinusoidal endothelium for facilitating cell entry into liver plates, and to accelerate cell integrations into liver parenchyma will advance applications of hepatocyte transplantation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 11:40:52