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Titolo:
Expression of vascular endothelial growth factor promotes colonization, vascularization, and growth of transplanted hepatic tissues in the mouse
Autore:
Ajioka, I; Akaike, T; Watanabe, Y;
Indirizzi:
Tokyopanst Technol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 226, Ja Tokyo Inst Technol Yokohama Kanagawa Japan 226 Yokohama, Kanagawa 226, Ja
Titolo Testata:
HEPATOLOGY
fascicolo: 2, volume: 29, anno: 1999,
pagine: 396 - 402
SICI:
0270-9139(199902)29:2<396:EOVEGF>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROCARRIER-ATTACHED HEPATOCYTES; ACUTE LIVER-FAILURE; PERMEABILITY FACTOR; BLOOD-VESSELS; TUMOR-GROWTH; ANGIOGENESIS; CELLS; RATS; SURVIVAL; DIFFERENTIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Watanabe, Y TokyoKanagawachnol, Dept Biomol Engn, Midori Ku, 4259 Nagatsuda, Yokohama, Tokyo Inst Technol 4259 Nagatsuda Yokohama Kanagawa Japan 226
Citazione:
I. Ajioka et al., "Expression of vascular endothelial growth factor promotes colonization, vascularization, and growth of transplanted hepatic tissues in the mouse", HEPATOLOGY, 29(2), 1999, pp. 396-402

Abstract

A complex vascular network forms an important component of the liver architecture. This network is essential for the supply of oxygen and nutrients to cells and delivery of molecules for metabolic exchange. In this study, weattempted to construct a vascular network in transplanted hepatic tissues and examined the effect of such network on tissue formation. Primary hepatocytes of adult mice were transfected with vascular endothelial growth factor (VEGF) gene in vitro then transplanted with collagen beads intraperitoneally in mice. VEGF-transfected hepatocytes secreted sufficient protein of the transgene in vitro to induce proliferation of endothelial cells. In vivo,VEGF-transfected hepatocytes formed a large number of colonies and developed a significant vascular network in established tissues compared with control tissues. In addition, hepatocytes of VEGF-transfected, established tissues proliferated and formed a substantial parenchymal region. These hepatocytes were also functional as confirmed by the production of albumin. Our results suggested that VEGF expression conferred not only the formation of a vascular network but also promoted tissue formation. Our study showed that ex vivo gene transfection into hepatocytes is a useful method for the induction of liver reconstitution in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/21 alle ore 14:59:18