Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cloning, expression and functional role of a nociceptin/orphanin FQ receptor in the porcine gastrointestinal tract
Autore:
Osinski, MA; Pampusch, MS; Murtaugh, MP; Brown, DR;
Indirizzi:
Univ Minnesota, Dept Vet Pathobiol, St Paul, MN 55105 USA Univ Minnesota St Paul MN USA 55105 Vet Pathobiol, St Paul, MN 55105 USA
Titolo Testata:
EUROPEAN JOURNAL OF PHARMACOLOGY
fascicolo: 2-3, volume: 365, anno: 1999,
pagine: 281 - 289
SICI:
0014-2999(19990122)365:2-3<281:CEAFRO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORPHANIN-FQ; OPIOID RECEPTOR; TISSUE DISTRIBUTION; OPIATE RECEPTORS; DISTAL JEJUNUM; ION-TRANSPORT; CDNA CLONING; MEMBER; FAMILY; LOCALIZATION;
Keywords:
myenteric plexus; 'orphan' opioid receptor; species homology; mRNA expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Brown, DR Univ Minnesota, Dept Vet Pathobiol, 1988 Fitch Ave, St Paul, MN 55105 USA Univ Minnesota 1988 Fitch Ave St Paul MN USA 55105 MN 55105 USA
Citazione:
M.A. Osinski et al., "Cloning, expression and functional role of a nociceptin/orphanin FQ receptor in the porcine gastrointestinal tract", EUR J PHARM, 365(2-3), 1999, pp. 281-289

Abstract

The heptadecapeptide nociceptin/orphanin FQ is the cognate ligand for the opioid receptor-like orphanin FQ (OFQ) receptor, a member of the G protein-coupled receptor superfamily. The gastrointestinal tract is a major site ofopioid action, and preliminary evidence suggests that an OFQ receptor may be expressed in rat small intestine. We addressed the hypothesis that this receptor is expressed in the gastrointestinal tract of the pig, a model forthe human digestive system. A 1205-bp cDNA was isolated from porcine forebrain which contained the 370 amino acid open reading frame encoding the OFQreceptor. The receptor mRNA is likely to arise from a single gene, as determined by Southern blotting of porcine genomic DNA restriction digests using a porcine OFQ receptor cDNA probe. A semi-nested reverse transcriptase-polymerase chain reaction survey of receptor mRNA indicates that it is expressed in the porcine cerebral cortex and kidney, and along the length of the gastrointestinal tract. OFQ decreased initial contractile responses of porcine ileal smooth muscle strips to trains of electrical field stimulation with an IC50 value of 1.3 nM; its effects were resistant to the opioid antagonist, naloxone. The peptide, at concentrations greater than or equal to 3 nM, also attenuated I-sc elevations evoked by electrical transmural stimulation of mucosa-submucosa sheets from porcine ileum. The actions of OFQ appeared to differ from those previously reported for opioid receptor agonists in these tissue preparations. These results indicate that an OFQ receptor isexpressed in the porcine intestine which modulates the neural control of intestinal smooth muscle contractility and mucosal transport. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 09:26:58