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Titolo:
In vitro testing of tensides employing monolayer cultures: a comparison with results of patch tests on human volunteers
Autore:
Benassi, L; Bertzzoni, G; Seidenari, S;
Indirizzi:
Univ Modena, Dept Dermatol, I-41100 Modena, Italy Univ Modena Modena Italy I-41100 a, Dept Dermatol, I-41100 Modena, Italy
Titolo Testata:
CONTACT DERMATITIS
fascicolo: 1, volume: 40, anno: 1999,
pagine: 38 - 44
SICI:
0105-1873(199901)40:1<38:IVTOTE>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SODIUM LAURYL SULFATE; NEUTRAL RED; SKIN IRRITATION; CELL-CULTURES; MODEL; KERATINOCYTES; CYTOTOXICITY; IRRITANTS; ASSAYS; PROLIFERATION;
Keywords:
emulsifier; tenside; surfactant; monolayer culture; in vitro tests; human patch tests; cytotoxicity; transepidermal water loss; colorimetry; skin hydration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Seidenari, S Univ Modena, Dept Dermatol, Via del Pozzo 71, I-41100 Modena,Italy Univ Modena Via del Pozzo 71 Modena Italy I-41100 ena, Italy
Citazione:
L. Benassi et al., "In vitro testing of tensides employing monolayer cultures: a comparison with results of patch tests on human volunteers", CONTACT DER, 40(1), 1999, pp. 38-44

Abstract

Evaluation of the irritant potential of new products or ingredients prior to human resting is generally performed in vivo on animals. However, according to the 6th amendment and following updates of the European Community directive on cosmetic products (93/35/EEC), animal testing will be banned when suitable substitutes will be available. To know whether in vitro tests for assessment of skin irritancy provide results approaching human conditions, comparisons have to be made between data deriving from in vitro tests andskin response in humans. The aim of our study was to assess the validity of the monolayer culture system of normal human keratinocytes as a model forthe evaluation of the irritant effects of detergents, by comparing in vitro cell culture data to in vivo acute skin irritancy effects of cocamidopropyl betaine (CAPB), an amphoteric compound, Tween 20 (TW20) (polysorbate 20)and Tween 80 (TW80) (polysorbate 80), representing nonionic compounds, applied to the skin of 24 healthy volunteers at a concentration similar to that employed in commercial products. As parameters for cytotoxicity, cell proliferation, cell membrane integrity and cell metabolism were assessed by cell counts, thymidine incorporation, MTT conversion, and Neutral Red uptake. In order to increase the sensitivity of the in vivo evaluation, bioengineering methods for assessment of the effects of test products on the skin were employed. Whereas all 4 in vitro methods ranked the tensides according totheir toxicity in the following order: CAPB>SLS>TW20>TW80, both in vivo methods agreed in identifying SLS as the most irritating substance. Moreover,as compared with the irritation potential on human skin, all 4 in vitro tests overestimated the toxicity of CAPB. This suggests that the keratinocytemonolayer cell culture technique cannot directly replace in vivo methods, and that data obtained by this method should be interpreted cautiously.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 14:42:48