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Titolo:
Development and validation of an HPLC method for the simultaneous determination of clozapine and desmethylclozapine in plasma of schizophrenic patients
Autore:
Raggi, MA; Bugamelli, F; Mandrioli, R; De Ronchi, D; Volterra, V;
Indirizzi:
Univ Bologna, Dept Pharmaceut Sci, I-40126 Bologna, Italy Univ Bologna Bologna Italy I-40126 harmaceut Sci, I-40126 Bologna, Italy Univ Bologna, Inst Psychiat, I-40126 Bologna, Italy Univ Bologna BolognaItaly I-40126 Inst Psychiat, I-40126 Bologna, Italy
Titolo Testata:
CHROMATOGRAPHIA
fascicolo: 1-2, volume: 49, anno: 1999,
pagine: 75 - 80
SICI:
0009-5893(199901)49:1-2<75:DAVOAH>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; SOLID-PHASE EXTRACTION; ULTRAVIOLET DETECTION; MAJOR METABOLITES; HUMAN SERUM; N-DESMETHYLCLOZAPINE; NORCLOZAPINE; ASSAY;
Keywords:
column liquid chromatography; amperometric detection; clozapine; desmethylclozapine; drug monitoring;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Raggi, MA Univ Bologna, Dept Pharmaceut Sci, Via Belmeloro 6, I-40126 Bologna, Italy Univ Bologna Via Belmeloro 6 Bologna Italy I-40126 logna, Italy
Citazione:
M.A. Raggi et al., "Development and validation of an HPLC method for the simultaneous determination of clozapine and desmethylclozapine in plasma of schizophrenic patients", CHROMATOGR, 49(1-2), 1999, pp. 75-80

Abstract

A high-performance liquid chromatographic method with amperometric detection has been developed for the determination of levels of clozapine (CLZ) and its active metabolite N-desmethylclozapine (DMC) in human plasma. The analysis was performed on a 5 mu m C8 reversed phase column (150 x 4.6 mm i.d,), with acetonitrile-phosphate buffer (pH 3.5), as the mobile phase. The detection voltage was +800 mV and the cell and column temperature were 50 degrees C. Linear responses were obtained between 2 ng mL(-1) and 100 ng mL(-1). Absolute recovery for both clozapine and desmethylclozapine exceeded 88%and the detection limit was 1 ng mL(-1). Repeatability, intermediate precision and accuracy were satisfactory. The method, which is rapid, sensitive and selective, has been applied to therapeutic drug monitoring in schizophrenic patients following administration of Leponex(R) tablets. In 21 patients in steady stare at a mean daily clozapine dosage of 358 mg (ranging from 150 to 500 mg day(-1)), clozapine levels averaged 379 ng mL(-1) (ranging from 102 to 818 ng mL(-1)) and DMC levels averaged 233 ng mL(-1) (ranging from 70 to 540 ng mL(-1)). The method requires only a very small amount of plasma (100 mu L), and thus it is suitable for pharmacokinetic studies, as well as for therapeutic drug monitoring.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/20 alle ore 01:06:42