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Titolo:
Immunohistochemical evidence for the presence, localization and partial coexistence of insulin, insulin-like growth factor I and relaxin in the protochordate Ciona intestinalis
Autore:
Reinecke, M; Eppler, E; David, I; Georges, D;
Indirizzi:
Univ Zurich, Inst Anat, Div Neuroendocrinol, CH-8057 Zurich, Switzerland Univ Zurich Zurich Switzerland CH-8057 inol, CH-8057 Zurich, Switzerland Univ Sci & Med Grenoble, Lab Biol Anim, Grenoble, France Univ Sci & Med Grenoble Grenoble France Lab Biol Anim, Grenoble, France
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 2, volume: 295, anno: 1999,
pagine: 331 - 338
SICI:
0302-766X(199902)295:2<331:IEFTPL>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLASSICAL ISLET HORMONES; GENE-EXPRESSION; IGF-I; BIOLOGICAL ACTIONS; BINDING-PROTEINS; LAYING HEN; OVARY; SYSTEM; SUPERFAMILY; RECEPTORS;
Keywords:
insulin; insulin-like growth factor I; relaxin; co-existence; ovary; digestive tract; protochordate; Ciona intestinalis (Tunicata);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Reinecke, M Univurich,h, Inst Anat, Div Neuroendocrinol, Winterthurerstr 190, CH-8057 Z Univ Zurich Winterthurerstr 190 Zurich Switzerland CH-8057 Z
Citazione:
M. Reinecke et al., "Immunohistochemical evidence for the presence, localization and partial coexistence of insulin, insulin-like growth factor I and relaxin in the protochordate Ciona intestinalis", CELL TIS RE, 295(2), 1999, pp. 331-338

Abstract

The occurrence and coexistence of peptides of the insulin-like growth factor (IGF)/insulin superfamily were investigated in the ovary and gastro-intestinal tract of the protochordate Ciona intestinalis. Antisera specific formammalian TGF-I, insulin and relaxin were used in a double-immunofluorescence method on paraffin sections and with an immunogold technique on consecutive semi-thin sections. IGF-I and relaxin immunoreactions but no insulin immunoreactions occurred in the ovary and were confined to medium-sized and mature follicle cells. Two subpopulations of reacting follicular cells werepresent: those containing only IGF-I immunoreactivity (5%) and those containing IGF-I and relaxin immunoreactivities (95%). In the gastrointestinal tract, IGF-I and insulin immunoreactions coexisted, whereas no relaxin immunoreactions were obtained. Gel chromatography and radioimmunoassay in Ciona ovary revealed IGF-I immunoreactivity in two peaks with apparent molecular masses of approximately 16 kDa and 3 kDa. The present results indicate that(1) the same IGF-I-related peptide probably occurs in gastro-intestinal tract and ovary, (2) three different members of the insulin/IGF family of peptides are probably present in protochordates, (3) different types of coexistence of these peptides seem to exist in protochordates, i.e. an IGF-I-related peptide and an insulin-related peptide in the digestive tract and, as shown previously, in central nervous system, and the IGF-I-related peptide and relaxin in the ovary, (4) an IGF-I-related peptide and relaxin may be involved in oocyte maturation in the protochordate ovary.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:55:06