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Titolo:
Cytochemical localization of GalNAc and GalNAc beta 1, 4Gal beta 1,4 disaccharide in mouse zona pellucida
Autore:
Aviles, M; Castells, MT; Abascal, I; Martinez-Menarguez, JA; Draber, P; Kan, FWK; Ballesta, J;
Indirizzi:
Univ Murcia, Sch Med, Dept Cell Biol, E-30071 Murcia, Spain Univ Murcia Murcia Spain E-30071 , Dept Cell Biol, E-30071 Murcia, Spain Acad Sci Czech Republ, Inst Mol Genet, Prague, Czech Republic Acad Sci Czech Republ Prague Czech Republic net, Prague, Czech Republic Queens Univ, Fac Med, Dept Anat & Cell Biol, Kingston, ON, Canada Queens Univ Kingston ON Canada pt Anat & Cell Biol, Kingston, ON, Canada
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 2, volume: 295, anno: 1999,
pagine: 269 - 277
SICI:
0302-766X(199902)295:2<269:CLOGAG>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
O-LINKED OLIGOSACCHARIDES; SPERM RECEPTOR ACTIVITY; ACETYL-D-GALACTOSAMINE; CARBOHYDRATE RESIDUES; MAMMALIAN FERTILIZATION; GLYCOPROTEIN ZP3; IN-VITRO; BINDING; EGGS; RAT;
Keywords:
cytochemistry; lectins; zona pellucida; oocyte; mouse (CD1);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Ballesta, J Univ Murcia, Sch Med, Dept Cell Biol, E-30071 Murcia, Spain Univ Murcia Murcia Spain E-30071 Biol, E-30071 Murcia, Spain
Citazione:
M. Aviles et al., "Cytochemical localization of GalNAc and GalNAc beta 1, 4Gal beta 1,4 disaccharide in mouse zona pellucida", CELL TIS RE, 295(2), 1999, pp. 269-277

Abstract

Carbohydrate residues contained in the zona pellucida play a key role in the process of sperm-egg interaction. In vitro fertilization experiments have shown that a specific monoclonal antibody against GalNAc beta 1,4Gal beta1,4 disaccharide inhibits fertilization in mice. In the present study, theultrastructural cytochemical localization of GalNAc residues and the GalNAc beta 1,4Gal beta 1,4, disaccharide was carried out in ovarian and postovulatory oocytes by using lectin-gold cytochemistry and immunocytochemistry. Plant lectins SEA and DBA showed an affinity for the entire zona pellucida matrix of ovarian oocytes throughout the follicular maturation; however, immunoreactivity for GalNAc beta 1,4Gal beta 1,4 disaccharide was not detected in ovarian oocytes at the earliest stages of follicular development but was found to be associated with the inner region of the zona matrix at the trilaminar primary follicle stage. The Golgi apparatus, vesicular aggregates, and cortical granules of the oocyte were intensely labeled by SEA and DBAthroughout follicular development. Immunoreactivity to GalNAc beta 1,4Gal beta 1,4 disaccharide was first observed in the Golgi apparatus and vesicular aggregates in trilaminar primary follicles. No immunoreactivity was observed in the cortical granules. In postovulatory oocytes, results were similar to those observed in ovarian oocytes. Our results thus suggest that (1) GalNAc beta 1,4Gal beta 1,4 disaccharide residues are present only in the inner region of the zona pellucida and, therefore, might be involved in sperm penetration through the zona pellucida, (2) the inner and outer regions of the zona pellucida contain different oligosaccharide chains, (3) the vesicular aggregates detected in the oocyte could represent an intermediate step in the secretory pathway of zona pellucida glycoproteins and might be involved in the formation of cortical granules.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 19:08:52