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Titolo:
Source of elastin-degrading enzymes in mycotic aortic aneurysms: bacteria or host inflammatory response?
Autore:
Buckmaster, MJ; Curci, JA; Murray, PR; Liao, S; Allen, BT; Sicard, GA; Thompson, RW;
Indirizzi:
Washington Univ, Sch Med, Dept Surg, Vasc Surg Sect, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 c Surg Sect, St Louis, MO 63110 USA Washington Univ, Sch Med, Dept Clin Microbiol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 n Microbiol, St Louis, MO 63110 USA Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ St Louis MO USA 63110 l & Physiol, St Louis, MO 63110 USA
Titolo Testata:
CARDIOVASCULAR SURGERY
fascicolo: 1, volume: 7, anno: 1999,
pagine: 16 - 26
SICI:
0967-2109(199901)7:1<16:SOEEIM>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PSEUDOMONAS-AERUGINOSA ELASTASE; SUPRARENAL ABDOMINAL-AORTA; MATRIX METALLOPROTEINASES; CYSTIC-FIBROSIS; ALPHA-1-PROTEINASE INHIBITOR; MECHANICAL-PROPERTIES; PATHOGENESIS; PURIFICATION; DEGRADATION; LASA;
Keywords:
elastin; elastases; mycotic aortic aneurysm; Pseudomonas aeruginosa; polymorphonuclear neutrophils; human neutrophil elastase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Buckmaster, MJ Washington Univ, Sch Med, Dept Surg, Vasc Surg Sect, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 Louis, MO 63110 USA
Citazione:
M.J. Buckmaster et al., "Source of elastin-degrading enzymes in mycotic aortic aneurysms: bacteria or host inflammatory response?", CARDIOV SUR, 7(1), 1999, pp. 16-26

Abstract

Elastolytic matrix metalloproteinases play a central role in the development of chronic atherosclerotic aortic aneurysms, but mycotic aortic aneurysms are a distinct and unusual form of aneurysm disease caused by bacterial infection. Mycotic aortic aneurysms follow a more rapid and unpredictable course than chronic aneurysm disease and they exhibit a predilection for the suprarenal aorta, further implying unique pathophysiologic mechanisms. The purpose of this study was to examine the nature and source of elastin-degrading enzymes in mycotic aortic aneurysm. Bacterial isolates and aortic tissues were obtained from four consecutive patients undergoing surgical repairof suprarenal mycotic aortic aneurysm. Using an In vitro H-3-labeled elastin degradation assay, elastin-degrading enzyme activity was only observed in the bacteria-conditioned medium from an isolate of Pseudomonas aeruginosa. Elastin-degrading enzyme activity in the aortic tissue homogenate of thispatient was abolished by the serine protease inhibitor, phenylmethylsulfonyl fluoride, but it was not suppressed by the metalloproteinase inhibitor, ethylenediamine tetraacetic acid (EDTA), In contrast, elastin-degrading enzyme activity in the bacterial-conditioned medium was decreased by about half by both phenylmethylsulfonyl fluoride and EDTA. Elastin substrate zymography revealed two phenylmethylsulfonyl fluoride-inhibitable elastin-degrading enzyme activities in the aortic tissue homogenate that corresponded to human neutrophil elastase (similar to 30 kDa) and its stable complex with alpha 1-proteinase inhibitor (similar to 80 kDa), but no activity attributableto Pseudomonas elastase, a 33-kDa metal-dependent enzyme. Human neutrophilelastase was readily detected throughout mycotic aortic aneurysm tissues by immunohistochemistry, but elastolytic metalloproteinases were only occasionally observed. The results of this study suggest that the elastin-degrading enzyme produced in mycotic aortic aneurysm are largely serine proteases of host neutrophil origin, rather than elastases produced by the infecting microorganisms or the macrophage-derived metalloproteinases typically observed in atherosclerotic aneurysm disease. Further studies will be needed to extend these findings to a larger number of patients with mycotic aortic aneurysm and those caused by additional microorganisms, (C) 1998 The International Society for Cardiovascular Surgery, Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 13:42:24