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Titolo:
Direct diagnosis of Chlamydia trachomatis genital tract infections: culture or PCR?
Autore:
Labau, E; Henry, S; Bennet, P; Massip, P; Chabanon, G;
Indirizzi:
CHU Purpan, Serv Malad Infect, Toulouse, France CHU Purpan Toulouse France Purpan, Serv Malad Infect, Toulouse, France CHU Rangueil, Virol Lab, Toulouse, France CHU Rangueil Toulouse FranceCHU Rangueil, Virol Lab, Toulouse, France Lab Tounis, Toulouse, France Lab Tounis Toulouse FranceLab Tounis, Toulouse, France
Titolo Testata:
PATHOLOGIE BIOLOGIE
fascicolo: 10, volume: 46, anno: 1998,
pagine: 813 - 818
SICI:
0369-8114(199812)46:10<813:DDOCTG>2.0.ZU;2-R
Fonte:
ISI
Lingua:
FRE
Soggetto:
POLYMERASE CHAIN-REACTION; CELL-CULTURE; SPECIMENS; ASSAY;
Keywords:
Chlamydia trachomatis; culture; gene amplification; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Labau, E CHU Purpan, Serv Malad Infect, Toulouse, France CHU Purpan Toulouse France Serv Malad Infect, Toulouse, France
Citazione:
E. Labau et al., "Direct diagnosis of Chlamydia trachomatis genital tract infections: culture or PCR?", PATH BIOL, 46(10), 1998, pp. 813-818

Abstract

PCR and culturing were compared for the routine diagnosis of Chlamydia trachomatis infections. Two laboratories experienced in both techniques participated in the study, which included 513 specimens. Both techniques were performed on each specimen; the portion of the specimen used for PCR was divided in two, and each half was sent to one of the two laboratories, where thetests were run in a blinded fashion. The PCR primers used by the two laboratories matched different parts of the bacterial genome. PCR inhibitors were looked for in all specimens. Overall, PCR was more sensitive than culturing; the difference was marked for sperm and endopelvic specimens and nonsignificant for urethral and cervical specimens. False-positive PCR results were few in number, there were no consistent false-positive results when eachspecimen was amplified twice. PCR inhibitors were rarely present in urethral and cervical specimens but were found in 7% of sperm and endopelvic specimens. PCR inhibitors should be looked for routinely during PCR testing of sperm or endopelvic specimens.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:33:12