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Titolo:
Analysis of cell cycle arrest in adipocyte differentiation
Autore:
Reichert, M; Eick, D;
Indirizzi:
GSF Forschungszentrum Umwelt & Gesundheit, Inst Klin Mol Biol & Tumorgenet, GSF Forschungszentrum Umwelt & Gesundheit Munich Germany D-81377 rgenet,
Titolo Testata:
ONCOGENE
fascicolo: 2, volume: 18, anno: 1999,
pagine: 459 - 466
SICI:
0950-9232(19990114)18:2<459:AOCCAI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
LARGE T-ANTIGEN; BINDING-PROTEIN-ALPHA; MYC-INDUCED APOPTOSIS; LARGE TUMOR-ANTIGEN; SV40 LARGE-T; C-MYC; DNA-REPLICATION; C/EBP-ALPHA; DEPENDENT KINASES; TRANSCRIPTIONAL REGULATION;
Keywords:
3T3-L1 cells; differentiation; adipocytes; cell cycle; Rb; SV40 large T antigen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Eick, D GSF Forschungszentrum Umwelt & Gesundheit, Inst Klin Mol Biol & Tumorgenet, GSF Forschungszentrum Umwelt & Gesundheit Marchioninistr 25 Munich Germany D-81377
Citazione:
M. Reichert e D. Eick, "Analysis of cell cycle arrest in adipocyte differentiation", ONCOGENE, 18(2), 1999, pp. 459-466

Abstract

Confluent 3T3-L1 preadipocytes differentiate to adipocytes in the presenceof insulin, dexamethasone, and isobutylmethylxanthine (IDI), A transient increase of DNA synthesis is induced in 3T3-L1 cells 18 h after addition of IDI, followed by an arrest in the G1 phase of the cell cycle. Growth arrested cells express the protooncogene oncogene c-myc and gene for the CCAAT/enhancer binding protein (C/EBP alpha) between day 2 and 5, While c-Myc is strongly implicated in cell proliferation, C/EBP alpha is a differentiation-specific transcription factor with antiproliferative, activity. Here we havecharacterized the cell cycle arrest in differentiating 3T3-L1 cells. Arrested cells express the Cdk inhibitors p21 and p27, but, at the same time, show hyperphosphorylation of Rb and expression of the E2F-regulated thymidinekinase gene. The addition of new serum to arrested cells resulted in cyclin A expression and Cdk2 activity, but not in DNA synthesis, Simian virus 40large tumor antigen (LTAg) is a potent mitogen, The mutant LTAg-K1, deficient in binding of pocket proteins and unable to induce DNA synthesis in serum-starved 3T3-L1 cells, efficiently induced DNA synthesis in differentiating 3T3-L1 cells. This indicates that pocket proteins are probably not in,ol,ed in the control of the cell cycle arrest during 3T3-L1 cell differentiation. Our data suggest that the differentiation-specific cell cycle block in3T3-L1 cells is resistant to high levels of c-Myc, inactivation of pocket proteins, upregulation of cyclin A levels, and Cdk2 activation, but can be abolished by a function of LTAg that is independent of binding to pocket proteins.

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Documento generato il 02/04/20 alle ore 09:45:35