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Titolo:
Glut1 and Glut3 expression, but not capillary density, is increased by cobalt chloride in rat cerebrum and retina
Autore:
Badr, GA; Zhang, JZ; Tang, J; Kern, TS; Ismail-Beigi, F;
Indirizzi:
Case Western Reserve Univ, Dept Med, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Case Western Reserve Univ, Dept Physiol & Biophys, Cleveland, OH 44106 USACase Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Case Western Reserve Univ, Diabet Res Ctr, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA
Titolo Testata:
MOLECULAR BRAIN RESEARCH
fascicolo: 1, volume: 64, anno: 1999,
pagine: 24 - 33
SICI:
0169-328X(19990122)64:1<24:GAGEBN>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOSE-TRANSPORTER EXPRESSION; ENDOTHELIAL GROWTH-FACTOR; GENE-EXPRESSION; OXIDATIVE-PHOSPHORYLATION; DIABETIC-RETINOPATHY; ISCHEMIC-INJURY; MESSENGER-RNA; HYPOXIA; CELLS; INHIBITION;
Keywords:
blood-brain barrier; blood-retinal barrier; Glut1 mRNA; Glut3 mRNA; competitive PCR; brain microvessel; retinal microvasculature; capillary density; hypoxia; VEGF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Ismail-Beigi, F Case Western Reserve Univ, Dept Med, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 4106 USA
Citazione:
G.A. Badr et al., "Glut1 and Glut3 expression, but not capillary density, is increased by cobalt chloride in rat cerebrum and retina", MOL BRAIN R, 64(1), 1999, pp. 24-33

Abstract

Treatment of rats with cobalt chloride [Co(LI)], an agent that stimulates the expression of a set of hypoxia-responsive genes, for 10-12 days resulted in 1.45- and 1.40-fold increases in the content of Glut1 mRNA and Glut1 in cerebral gray matter, respectively (P < 0.05 for both changes). The increase in Glut1 content was associated with a significant increase in the content of Glut1 staining in microvessels isolated from cerebral gray matter, and in the intensity of Glut1 in microvessels of the frontal lobe and hippocampus assessed by immunohistochemistry. The abundance of Glut3 in cerebrum of Co(II)-treated rats also increased by 1.3-fold(P < 0.05), but the increase was not associated with a change in the content of Glut3 mRNA. In retina, treatment with Co(II) resulted in 2.48- and 1.23-fold increases in the content of Glut1 mRNA and Glut1 protein, respectively (P < 0.05 for both changes); similar increases in Glut1 protein expression were observed in isolated retinal microvasculature. The content of Glut3 in retina also increased 1.5-fold in Co(II)-treated rats (P < 0.05). In addition, treatment with Co(II) resulted in a significant 2.2-fold increase in the expression of VEGF in the cerebrum. However, despite the Co(II)-induced increase in Glut1 expression in cerebral and retinal microvasculature and VEGF in cerebrum, there was no increase in the capillary density in either tissue. It is concluded that a 10-12 day exposure to Co(II), presumably acting through the hypoxia-signaling pathway, results in enhanced expression of both major glucose transporters in cerebral cortex and retina, without increasing the capillary density of either tissue. (C) 1999 Elsevier Science B.V. All rights reserved.

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Documento generato il 29/03/20 alle ore 09:35:21