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Titolo:
Neuronal soma-dendritic and prejunctional M-1-M-4 receptors in gastrointestinal and genitourinary smooth muscle
Autore:
Lambrecht, G; Gross, J; Mutschler, E;
Indirizzi:
Univanyankfurt, Bioctr Niederursel, Dept Pharmacol, D-60439 Frankfurt, Germ Univ Frankfurt Frankfurt Germany D-60439 rmacol, D-60439 Frankfurt, Germ
Titolo Testata:
LIFE SCIENCES
fascicolo: 6-7, volume: 64, anno: 1999,
pagine: 403 - 410
SICI:
0024-3205(19990108)64:6-7<403:NSAPMR>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
RABBIT VAS-DEFERENS; SELECTIVE MUSCARINIC AGONISTS; LOWER ESOPHAGEAL SPHINCTER; PERFUSED RAT STOMACH; GUINEA-PIG ILEUM; URINARY-BLADDER; IN-VITRO; H-3 ACETYLCHOLINE; ENDOGENOUS NORADRENALINE; MEDIATING INHIBITION;
Keywords:
smooth muscle; presynaptic muscarinic receptors; rabbit anococcygeus muscle; noradrenaline release;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Lambrecht, G Univ439ankfurt, Bioctr Niederursel, Dept Pharmacol, Marie Curie Str 9, D-60 Univ Frankfurt Marie Curie Str 9 Frankfurt Germany D-60439 0
Citazione:
G. Lambrecht et al., "Neuronal soma-dendritic and prejunctional M-1-M-4 receptors in gastrointestinal and genitourinary smooth muscle", LIFE SCI, 64(6-7), 1999, pp. 403-410

Abstract

A variety of neurons in gastrointestinal and genitourinary smooth muscle express muscarinic auto- as well as heteroreceptors. These receptors are found on the soma and dendrites of many cholinergic, sympathetic and NANC neurons and on axon terminals. A given neuron may contain both excitatory and inhibitory presynaptic muscarinic receptors. The subtypes involved are species- and tissue-dependent, and neuronal M-1 to M-4 receptors have been shownto be expressed in smooth muscle tissues. In this study, the ability of several selective muscarinic receptor antagonists to inhibit the effect of arecaidine propargyl ester (APE) on prejunctional muscarinic receptors on sympathetic nerve endings in the rabbit anococcygeus muscle (RAM) was investigated to characterise the receptor subtype involved. Electrical field stimulation (EFS) resulted in a release of noradrenaline (NA) eliciting monophasic contractions due to stimulation of postjunctional alpha(1)-adrenoceptors. The selective muscarinic agonist APE did not reduce contractions to exogenous NA, but caused a concentration-related and N-methylatropine-sensitive inhibition of neurogenic responses. All muscarinic antagonists investigated failed to affect the EFS-induced contractions, but shifted the concentration-response curve of APE to the right in a parallel and surmountable fashion. Schild analysis yielded regression lines of unit slope, indicating competitive antagonism. The following rank order of antagonist potencies (pA(2) values) was found: tripitramine (9.10) > AQ-RA 741 (8.26) greater than or equal to himbacine (8.04) greater than or equal to (S)-dimethindene (7.69)> pirenzepine (6.46)greater than or equal to p-F-HHSiD (6.27). A comparison ofthe pA(2) values determined in the present study with literature binding and functional affinities obtained at native or recombinant M-1 to M-5 receptors strongly suggests that NA release from sympathetic nerve endings in RAM is inhibited by activation of prejunctional muscarinic M-2 receptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 09:43:55