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Titolo:
Comparison of screening methods in detection of bladder cancer
Autore:
Ramakumar, S; Bhuiyan, J; Besse, JA; Roberts, SC; Wollan, PC; Blute, ML; OKane, DJ;
Indirizzi:
Mayo Clin & Mayo Grad Sch Med, Dept Urol, Rochester, MN 55901 USA Mayo Clin & Mayo Grad Sch Med Rochester MN USA 55901 hester, MN 55901 USA MayoSAlin & Mayo Grad Sch Med, Dept Lab Med & Pathol, Rochester, MN 55901 U Mayo Clin & Mayo Grad Sch Med Rochester MN USA 55901 ochester, MN 55901 U Mayo Clin & Mayo Grad Sch Med, Dept Biostat, Rochester, MN 55901 USA Mayo Clin & Mayo Grad Sch Med Rochester MN USA 55901 hester, MN 55901 USA
Titolo Testata:
JOURNAL OF UROLOGY
fascicolo: 2, volume: 161, anno: 1999,
pagine: 388 - 394
SICI:
0022-5347(199902)161:2<388:COSMID>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSITIONAL-CELL CANCER; NUCLEAR MATRIX PROTEIN; VOIDED URINE CYTOLOGY; BARD BTA TEST; TELOMERASE ACTIVITY; DEGRADATION PRODUCTS; WASH CYTOLOGY; CARCINOMA; DIAGNOSIS; NEOPLASMS;
Keywords:
bladder neoplasms; telomerase; tumor markers; biological; cytology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: O'Kane, DJ Mayo5905n & Mayo Fdn, Dept Lab Med & Pathol, 200 1st St SW, Rochester, MN 5 Mayo Clin & Mayo Fdn 200 1st St SW Rochester MN USA 55905 MN 5
Citazione:
S. Ramakumar et al., "Comparison of screening methods in detection of bladder cancer", J UROL, 161(2), 1999, pp. 388-394

Abstract

Purpose: We prospectively evaluate and compare the sensitivity and specificity of urine cytology, BTA stat,dagger NMP22,double dagger fibrin/fibrinogen degradation products (FDP), telomerase, chemiluminescent hemoglobin and hemoglobin dipstick to detect bladder cancer. Materials and Methods: Single voided specimens were obtained from 57 patients with bladder cancer, and 139 without evidence of bladder malignancy on cystoscopy or a negative biopsy of indeterminate lesions. A cytology reportwas available for 125 patients and interpreted independently . BTA slat, NMP22 and FDP were analyzed according to manufacturer specifications. The telomerase assay was performed on cells collected from urine by centrifugation in preparation for polymerase chain reaction based amplification using the telomeric repeat amplification protocol assay. The chemiluminescent screening assay for hemoglobin in urine uses the pseudoperoxidase activity of hemoglobin on hydrogen peroxide and subsequent oxidation of 7-dimethylaminonaphthalene-1,2-dicarbonic acid hydrazide to generate chemiluminescence emission. Hemoglobin dipstick was interpreted as positive if the hemoglobin content in the urine was trace or greater. Results: Overall sensitivity with urine cytology, BTA stat, NMP22, FDP, telomerase, chemiluminescent hemoglobin and the hemoglobin dipstick was 44, 74, 53, 52, 70, 67 and 47%, respectively. Specificity with cytology, telomerase and FDP was high (95, 99 and 91%, respectively) but BTA stat, NMP22 (optimized), chemiluminescent hemoglobin (optimized) and the hemoglobin dipstick demonstrated lower specificity of 73, 60, 63 and 84%, respectively. Stepwise logistic regression analysis revealed that for all tumors, and within each tumor grade and stage telomerase had the strongest association with bladder cancer among all tests (69% overall concordance). Telomerase was alsopositive in 91% of the patients (10 of 11) with carcinoma in situ. Conclusions: Urinary telomerase had the highest combination of sensitivityand specificity (70 and 99%, respectively) for bladder cancer screening inthese patients. It was the strongest predictor with superior accuracy in patients with grade 1 and noninvasive tumors (pTa), and extremely useful in patients with carcinoma in situ. Telomerase appears to be promising and outperformed cytology, BTA stat, NMP22, FDP, chemiluminescent hemoglobin and hemoglobin dipstick in the prediction of bladder cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 15:30:30