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Titolo:
A computer program to compare sequence fingerprints of homologous proteinsfor the rapid assessment of their primary structure differences
Autore:
Caporale, C; Sepe, C; Caruso, C; Buonocore, V;
Indirizzi:
Univ Tuscia, Dipartimento Agrobiol & Agrochim, I-01100 Viterbo, Italy UnivTuscia Viterbo Italy I-01100 iol & Agrochim, I-01100 Viterbo, Italy
Titolo Testata:
JOURNAL OF PROTEIN CHEMISTRY
fascicolo: 8, volume: 17, anno: 1998,
pagine: 867 - 873
SICI:
0277-8033(199811)17:8<867:ACPTCS>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
UNFRACTIONATED DIGESTION MIXTURES; MASS-SPECTROMETRIC DATA; AMINO-ACID-SEQUENCE; PEPTIDE MIXTURES; SPECTRAL DATA; IDENTIFICATION; DATABASES; ALGORITHM; ALIGNMENT; PROTEASES;
Keywords:
algorithm; computer; protein sequence; mass spectrometry; peptide mixtures;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Caporale, C Univ Tuscia, Dipartimento Agrobiol & Agrochim, I-01100 Viterbo, Italy Univ Tuscia Viterbo Italy I-01100 im, I-01100 Viterbo, Italy
Citazione:
C. Caporale et al., "A computer program to compare sequence fingerprints of homologous proteinsfor the rapid assessment of their primary structure differences", J PROTEIN C, 17(8), 1998, pp. 867-873

Abstract

We have developed a computer program for the rapid assessment of the primary structure differences between a protein of unknown sequence and a homologous known protein. Both proteins are reduced, alkylated, and digested withthe same hydrolytic agent. The unfractionated peptide mixtures are submitted to automatic sequence analysis. Based on the knowledge of the reference sequence, the program utilizes the analysis data to identify all the potential peptides present in the two mixtures, determining their primary structure, homology degree, and molecular weight calculated both as integer MH+ and average mass variables. These fingerprints allow the user to easily identify the structural differences between the mio proteins and clarify possible doubts by a mass spectrometric analysis of the two mixtures. In order to verify the utility of the program, we provide an application example using the already reported data of two homologous proteins.

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Documento generato il 22/10/20 alle ore 03:32:20