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Titolo:
Nuclear localization of the delta subunit of Ca2+/calmodulin-dependent protein kinase II in rat cerebellar granule cells
Autore:
Takeuchi, Y; Yamamoto, H; Matsumoto, K; Kimura, T; Katsuragi, S; Miyakawa, T; Miyamoto, E;
Indirizzi:
Kumamoto Univ, Sch Med, Dept Pharmacol, Kumamoto 8600811, Japan Kumamoto Univ Kumamoto Japan 8600811 Pharmacol, Kumamoto 8600811, Japan Kumamoto Univ, Sch Med, Dept Neuropsychiat, Kumamoto 8600811, Japan Kumamoto Univ Kumamoto Japan 8600811 ropsychiat, Kumamoto 8600811, Japan
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 2, volume: 72, anno: 1999,
pagine: 815 - 825
SICI:
0022-3042(199902)72:2<815:NLOTDS>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAM KINASE; MOLECULAR-CLONING; MESSENGER-RNAS; CALMODULIN; BRAIN; EXPRESSION; ACTIVATION; ISOFORMS; GAMMA; TRANSCRIPTION;
Keywords:
calmodulin; Ca2+/camodulin-dependent protein kinase II; cerebellum; granule cells; nucleus; overexpression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Yamamoto, H KumamotonUniv, Sch Med, Dept Pharmacol, 2-2-1 Honjo, Kumamoto 8600811, Japa Kumamoto Univ 2-2-1 Honjo Kumamoto Japan 8600811 600811, Japa
Citazione:
Y. Takeuchi et al., "Nuclear localization of the delta subunit of Ca2+/calmodulin-dependent protein kinase II in rat cerebellar granule cells", J NEUROCHEM, 72(2), 1999, pp. 815-825

Abstract

To examine the physiological roles of the delta subunit of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II delta) in brain, we examined the localization of CaM kinase II delta in the rat brain. A specific antibodyto CaM kinase II delta 1-delta 4 isoforms was prepared by immunizing rabbits with a synthesized peptide corresponding to the unique carboxyl-terminalend of these isoforms. The prepared antibody did not recognize the alpha, beta, and gamma subunits, which were each overexpressed in NG108-15 cells. Immunoblot analysis on Various regions and the nuclear fractions from rat brains suggested that some isoforms of CaM kinase II delta 1-delta 4 were abundant in the nucleus in the cerebellum. Total RNA from the cerebellum was analyzed by RT-PCR with a primer pair from variable domain 1 to variable domain 2. We detected the three PCR products delta 3.1, delta 3.4, and delta 3 that contained the nuclear localization signal. These CaM kinase II delta3 isoforms were localized in the nuclei in transfected NG108-15 cells. Immunohistochemical study suggested the existence of these isoforms in the nuclei in cerebellar granule cells. These results suggest that CaM kinase II delta 3 isoforms are involved in nuclear Ca2+ signaling in cerebellar granule cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:30:56