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Titolo:
Identification of HLA-A3-restricted cytotoxic T lymphocyte epitopes from carcinoembryonic antigen and HER-2/neu by primary in vitro immunization withpeptide-pulsed dendritic cells
Autore:
Kawashima, I; Tsai, V; Southwood, S; Takesako, K; Sette, A; Celis, E;
Indirizzi:
Mayo Clin & Mayo Fdn, Dept Immunol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 unol, Rochester, MN 55905 USA Takara Shuzo Co Ltd, Biotechnol Res Labs, Otsu, Shiga 52021, Japan Takara Shuzo Co Ltd Otsu Shiga Japan 52021 Labs, Otsu, Shiga 52021, Japan Epimmune Inc, San Diego, CA 92121 USA Epimmune Inc San Diego CA USA 92121Epimmune Inc, San Diego, CA 92121 USA
Titolo Testata:
CANCER RESEARCH
fascicolo: 2, volume: 59, anno: 1999,
pagine: 431 - 435
SICI:
0008-5472(19990115)59:2<431:IOHCTL>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR RECEPTOR; NEU ONCOGENE; LUNG ADENOCARCINOMAS; GENE AMPLIFICATION; OVARIAN-CANCER; HUMAN-MELANOMA; BREAST-CANCER; CTL EPITOPES; EXPRESSION; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Celis, E Mayo Clin & Mayo Fdn, Dept Immunol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 hester, MN 55905 USA
Citazione:
I. Kawashima et al., "Identification of HLA-A3-restricted cytotoxic T lymphocyte epitopes from carcinoembryonic antigen and HER-2/neu by primary in vitro immunization withpeptide-pulsed dendritic cells", CANCER RES, 59(2), 1999, pp. 431-435

Abstract

The human carcinoembryonic antigen (CEA) and HER-2/neu are potential target antigens for CTL specific immunotherapy for common malignancies such as breast, lung, colon, and gastric carcinomas. Several CTL epitopes restrictedby HLA-AZ, the mast common human histocompatibility molecule, have been previously reported. However, to develop CTL-based immunotherapies for the general population, it is necessary to identify epitopes restricted by other common histocompatibility alleles. Here, we describe two HLA-A3-restricted CTL epitopes from the CEA and HER-2/neu antigens. HLA-A3 binding synthetic peptides from CEA and HER-2/neu were tested for immunogenicity by in vitro primary CTL induction protocol using peripheral blood mononuclear cells from normal healthy volunteers. One peptide from CEA (CEA[9(61)]: HLFGYSWYK) and one peptide from HER-2/neu (HER2[9(754)]: VLRENTSPK) were shown to induce CTL that was capable of killing a tumor cell line expressing HLA-A3 and the corresponding tumor-associated antigen. Additional NMC binding studies with the most common HLA molecules belonging to the HLA-A3 superfamily (HLA-A*1101, -A*3101, -A*3301, and -A*6801), demonstrated that CEA[9(61)] binds five of five A3 supertype molecules with high affinity, and the HER2[9(754)] epitope was able to bind to four of the same five alleles. These results indicate that these two new CTL epitopes should be inmunogenic in individuals expressing either HLA-A3, or other members of the HLA-A3 superfamily.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:49:32