Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Evaluation of polycyclic aromatic hydrocarbon-DNA adducts in exfoliated oral cells by an immunohistochemical assay
Autore:
Romano, G; Sgambato, A; Boninsegna, A; Flamini, G; Curigliano, G; Yang, Q; La Gioia, V; Signorelli, C; Ferro, A; Capelli, G; Santella, RM; Cittadini, A;
Indirizzi:
CatholicCtr,v Sacred Heart, Sch Med, Inst Gen Pathol, Giovanni 23 Res CancCatholic Univ Sacred Heart Rome Italy I-00168 thol, Giovanni 23 Res Canc Catholic Univ Sacred Heart, Sch Med, Inst Hyg, I-00168 Rome, Italy Catholic Univ Sacred Heart Rome Italy I-00168 t Hyg, I-00168 Rome, Italy Ufficio Capo Corpo Sanit Aeronaut, I-00185 Rome, Italy Ufficio Capo Corpo Sanit Aeronaut Rome Italy I-00185 I-00185 Rome, Italy Columbia Univ, Sch Publ Hlth, Div Environm Hlth Sci, New York, NY 10032 USA Columbia Univ New York NY USA 10032 ronm Hlth Sci, New York, NY 10032 USA
Titolo Testata:
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
fascicolo: 1, volume: 8, anno: 1999,
pagine: 91 - 96
SICI:
1055-9965(199901)8:1<91:EOPAHA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
P-32 POSTLABELING ANALYSIS; LUNG-CANCER PATIENTS; 4-AMINOBIPHENYL-DNA ADDUCTS; MUCOSAL CELLS; NONSMOKERS; SMOKERS; SUSCEPTIBILITY; SMOKING; QUANTITATION; DAMAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Romano, G CatholicCtr,v Sacred Heart, Sch Med, Inst Gen Pathol, Giovanni 23 Res Canc Catholic Univ Sacred Heart Largo Francesco Vito 1 Rome Italy I-00168
Citazione:
G. Romano et al., "Evaluation of polycyclic aromatic hydrocarbon-DNA adducts in exfoliated oral cells by an immunohistochemical assay", CANC EPID B, 8(1), 1999, pp. 91-96

Abstract

Polycyclic aromatic hydrocarbon-DNA adducts were evaluated in oral cells from 98 healthy volunteers by an immunohistochemical method using a specificantiserum against benzo(a)pyrene-DNA adducts revealed by the immunoperoxidase reaction. Mean adduct content, determined as relative staining intensity by absorbance image analyzer, was significantly higher in the cells from tobacco smokers compared with nonsmokers (330 +/- 98, n = 33 versus 286 +/-83, n = 64, respectively) with a P = 0.013 obtained by two-sample t test with equal variances, We found that in the smoker group, the PAH-DNA adduct content increases with the number of cigarettes. Thus, the relative staining intensity was 305 +/- 105 in the group smoking 1-10 cigarettes/day (n = 16), 347 +/- 77 in the 11-20 group (n = 14), and 386 +/- 112 in the group smoking more than 20 cigarettes/day (n = 3; P = 0.03 by nonparametric test for trend). No significant association was detected between PAH-DNA adducts in oral cells and variables such as residential area, oral infections, alcohol or vitamin intake, grilled food consumption, and professional activity, This work confirms and extends previous data suggesting that this immunohistochemical method might be used as a valuable dosimeter of genotoxic damagein a carcinogen-exposed population, although further studies are needed toverify the applicability of the test in high-risk populations other than smokers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 00:49:50