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Titolo:
In vivo isolated kidney perfusion with tumour necrosis factor alpha (TNF-alpha) in tumour-bearing rats
Autore:
van der Veen, AH; Seynhaeve, ALB; Breurs, J; Nooijen, PTGA; Marquet, RL; Eggermont, AMM;
Indirizzi:
Univterdam,dam Hosp, Dept Surg, Dr Daniel Den Hoed Canc Ctr, NL-3000 CA Rot Univ Rotterdam Hosp Rotterdam Netherlands NL-3000 CA Ctr, NL-3000 CA Rot Univ Nijmegen Hosp, Dept Pathol, Nijmegen, Netherlands Univ Nijmegen HospNijmegen Netherlands t Pathol, Nijmegen, Netherlands
Titolo Testata:
BRITISH JOURNAL OF CANCER
fascicolo: 3-4, volume: 79, anno: 1999,
pagine: 433 - 439
SICI:
0007-0920(199902)79:3-4<433:IVIKPW>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOLATED LIMB PERFUSION; ISOLATED HEPATIC PERFUSION; ISOLATED LUNG PERFUSION; RECOMBINANT HUMAN; INTERFERON-GAMMA; IFN-GAMMA; PHASE-I; MELPHALAN; MODEL; COMBINATION;
Keywords:
tumour necrosis factor alpha; isolated kidney perfusion; rats;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: van der Veen, AH Univ000tterdam Hosp, Dept Surg, Dr Daniel Den Hoed Canc Ctr, POB 2040, NL-3 Univ Rotterdam Hosp POB 2040 Rotterdam Netherlands NL-3000 CA
Citazione:
A.H. van der Veen et al., "In vivo isolated kidney perfusion with tumour necrosis factor alpha (TNF-alpha) in tumour-bearing rats", BR J CANC, 79(3-4), 1999, pp. 433-439

Abstract

Isolated perfusion of the extremities with high-dose tumour necrosis factor alpha (TNF-alpha) plus melphalan leads to dramatic tumour response in patients with irresectable soft tissue sarcoma or multiple melanoma in transitmetastases. We developed in vivo isolated organ perfusion models to determine whether similar tumour responses in solid organ tumours can be obtainedwith this regimen. Here, we describe the technique of isolated kidney perfusion. We studied the feasibility of a perfusion with TNF-alpha and assessed its anti-tumour effects in tumour models differing in tumour vasculature. The maximal tolerated dose (MTD) proved to be only 1 mu g TNF-alpha. Higher doses appeared to induce renal failure and a secondary cytokine release with fatal respiratory and septic shock-like symptoms. In vitro, the combination of TNF-alpha and melphalan did not result in a synergistic growth-inhibiting effect on CC 531 colon adenocarcinoma cells, whereas an additive effect was observed on osteosarcoma ROS-1 cells. In vivo isolated kidney perfusion, with TNF-alpha alone or in combination with melphalan, did not resultin a significant anti-tumour response in either tumour model in a subrenalcapsule assay. We conclude that, because of the susceptibility of the kidney to perfusion with TNF-alpha, the minimal threshold concentration of TNF-alpha to exert its anti-tumour effects was not reached. The applicability of TNF-alpha in isolated kidney perfusion for human tumours seems, therefore, questionable.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 23:29:39