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Titolo:
A disposable biosensor for urea determination in blood based on an ammonium-sensitive transducer
Autore:
Eggenstein, C; Borchardt, M; Diekmann, C; Grundig, B; Dumschat, C; Cammann, K; Knoll, M; Spener, F;
Indirizzi:
Inst Chemo & Biosensorik Munster, D-48149 Munster, Germany Inst Chemo & Biosensorik Munster Munster Germany D-48149 unster, Germany Univ Munster, Inst Biochem, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 t Biochem, D-48149 Munster, Germany
Titolo Testata:
BIOSENSORS & BIOELECTRONICS
fascicolo: 1, volume: 14, anno: 1999,
pagine: 33 - 41
SICI:
0956-5663(19990101)14:1<33:ADBFUD>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTRODE; SENSOR; SERUM;
Keywords:
disposable biosensor; disposable reference electrode; potentiometric biosensor; urea biosensor; blood urea;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Spener, F Inst Chemo & Biosensorik Munster, Mendelstr 7, D-48149 Munster, Germany Inst Chemo & Biosensorik Munster Mendelstr 7 Munster Germany D-48149
Citazione:
C. Eggenstein et al., "A disposable biosensor for urea determination in blood based on an ammonium-sensitive transducer", BIOSENS BIO, 14(1), 1999, pp. 33-41

Abstract

A potentiometric urea-sensitive biosensor using a NH4+-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is described. The ion-sensitive polymer matrix membrane was formed in the presence of an additional electrochemical inert filter paper matrix to improve thereproducibility in sensor production. The electrodes were prepared from one-side silver-coating filter paper, which is encapsulated for insulation bya heat-sealing film. Adefined volume of the NH4+-sensitive polymer matrix membrane cocktail was deposited on this filter paper. To obtain the urea-biosensor a layer of urease was cast onto the ion-sensitive membrane. Poly (carbamoylsulfonate) hydrogel, produced from a hydrophilic polyurethane prepolymer blocked with bisulfite, served as immobilisation material. The disposable urea-sensitive electrode was combined with a disposable Ag/AgCl reference electrode to obtain the disposable urea biosensor. The sensor respondedrapidly and in a stable manner to changes in urea concentrations between 7.2 x 10(-5) and 2.1 x 10(-2) mol/l. The detection limit was 2 x 10-5 mol/l urea and the slope in the linear range 52 mV/decade. By taking into consideration the influence of the interfering K+- and Na+- ions the sensor can beused for the determination of urea in human blood and serum samples (diluted or undiluted). A good correlation was found with the data obtained by the spectrophotometric routine method. (C) 1999 Elsevier Science S.A. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 22:43:47