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Titolo:
Investigation into the biological stability of collagen/chondroitin-6-sulphate gels and their contraction by fibroblasts and keratinocytes: the effect of crosslinking agents and diamines
Autore:
Osborne, CS; Reid, WH; Grant, MH;
Indirizzi:
Univndtrathclyde, Bioengn Unit, Wolfson Ctr, Glasgow G4 0NW, Lanark, Scotla Univ Strathclyde Glasgow Lanark Scotland G4 0NW ow G4 0NW, Lanark, Scotla
Titolo Testata:
BIOMATERIALS
fascicolo: 3, volume: 20, anno: 1999,
pagine: 283 - 290
SICI:
0142-9612(199902)20:3<283:IITBSO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
CROSS-LINKING AGENTS; COLLAGEN GEL; MECHANICAL FORCES; WOUND CONTRACTION; ENDOTHELIAL-CELLS; CULTURE; GROWTH; CYTOCOMPATIBILITY; GLUTARALDEHYDE; INVITRO;
Keywords:
artificial skin substitutes; collagen/chondroitin-6-sulphate gels; crosslinking agents; biological stability;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Grant, MH Univ Strathclyde, Bioengn Unit, Wolfson Ctr, 106 Rottenrow, Glasgow G4 0NW, Univ Strathclyde 106 Rottenrow Glasgow Lanark Scotland G4 0NW W,
Citazione:
C.S. Osborne et al., "Investigation into the biological stability of collagen/chondroitin-6-sulphate gels and their contraction by fibroblasts and keratinocytes: the effect of crosslinking agents and diamines", BIOMATERIAL, 20(3), 1999, pp. 283-290

Abstract

Artificial skin substitutes based on autologous keratinocytes cultured on collagen-based substrata are being developed for grafting onto patients with severe burns. The properties of the substratum can be manipulated by crosslinking the collagen with the glysocaminoglycan, chondroitin-6-sulphate (Ch6SO(4) ), carbodiimides and polyamines. Biological stability, assessed by resistance to collagenase, was increased by incorporation of Ch6SO(4) but crosslinking with the carbodiimides, 1-ethyl-3-(dimethylaminopropy1)carbodiimide and 1,1-carbonyldiimidazole or the polyamines, putrescine or diaminohexane, had little further benefit. Contraction of the collagen gels occurredto a greater extent when seeded with fibroblasts than with keratinocytes. The extent of contraction by either cell type was not influenced by the presence of Ch6SO(4) in the gel, but the carbodiimides, and to a lesser extentthe polyamines, limited cell-mediated contraction, particularly that mediated by fibroblasts. Optimum substratum composition for artificial skin substitutes will involve a compromise between the desired attributes of biological stability, rate of contraction, mechanical strength, biocompatibility and promotion of cell growth. (C) 1999 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 17:19:55