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Titolo:
Overexpression of the cytoplasmic retention signal region of cyclin B2, but not of cyclin B1, inhibits bipolar spindle formation in Xenopus oocytes
Autore:
Yoshitome, S; Furuno, N; Sagata, N;
Indirizzi:
Kyushu Univ, Fac Sci, Dept Biol, Fukuoka 8128581, Japan Kyushu Univ Fukuoka Japan 8128581 Sci, Dept Biol, Fukuoka 8128581, Japan
Titolo Testata:
BIOLOGY OF THE CELL
fascicolo: 6-7, volume: 90, anno: 1998,
pagine: 509 - 518
SICI:
0248-4900(199810)90:6-7<509:OOTCRS>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATURATION-PROMOTING FACTOR; PROTO-ONCOGENE PRODUCT; CELL-CYCLE; MEIOTIC MATURATION; A-TYPE; DEPENDENT KINASES; GOLGI-APPARATUS; MITOTIC SPINDLE; MESSENGER-RNA; M-PHASE;
Keywords:
bipolar spindle formation; cyclin B; subcellular localization; Xenopus oocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Sagata, N Kyushu Univ, Fac Sci, Dept Biol, Hakozaki 6-10-1, Fukuoka 8128581, Japan Kyushu Univ Hakozaki 6-10-1 Fukuoka Japan 8128581 128581, Japan
Citazione:
S. Yoshitome et al., "Overexpression of the cytoplasmic retention signal region of cyclin B2, but not of cyclin B1, inhibits bipolar spindle formation in Xenopus oocytes", BIO CELL, 90(6-7), 1998, pp. 509-518

Abstract

Cyclin B, a regulatory subunit of maturation/M-phase promoting factor (MPF), has several subtypes in many vertebrate species. However, it is not known whether the different B-type cyclins have any different functions in vertebrate cells, although their subcellular localizations seem to differ largely from each other. To examine the roles of two major B-type cyclins, B1 and B2, in spindle formation in M phase, we overexpressed their N-termini in Xenopus oocytes; the N-termini of cyclins B1 and B2 contained a cytoplasmicretention signal (CRS), and hence their overexpressions were expected to competitively inhibit the subcellular localizations of the endogenous cyclins B1 and B2, respectively. Upon entry into meiosis I, oocytes overexpressing the cyclin B1 N-terminus formed an apparently normal bipolar spindle, butthose oocytes overexpressing the cyclin B2 N-terminus formed a monopolar (or monoastral) spindle. This defect in bipolar spindle formation was observed only when the cyclin B2 N-terminus contained its own CRS sequence, and was able to be rescued by overexpression of full-length cyclin B2. These results suggest, for the first time, that the correct subcellular localizationof cyclin B2, but not of cyclin B1, is essential for (the initiation of) bipolar spindle formation in Xenopus oocytes. ((C) Elsevier, Paris).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:53:24