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Titolo:
Enzymes of porcine brain hydrolyzing 2-arachidonoylglycerol, an endogenousligand of cannabinoid receptors
Autore:
Goparaju, SK; Ueda, N; Taniguchi, K; Yamamoto, S;
Indirizzi:
Univ Tokushima, Dept Biochem, Sch Med, Tokushima 7708503, Japan Univ Tokushima Tokushima Japan 7708503 Sch Med, Tokushima 7708503, Japan
Titolo Testata:
BIOCHEMICAL PHARMACOLOGY
fascicolo: 4, volume: 57, anno: 1999,
pagine: 417 - 423
SICI:
0006-2952(19990215)57:4<417:EOPBH2>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOACYLGLYCEROL LIPASE; ANANDAMIDE AMIDOHYDROLASE; NG108-15 CELLS; RAT-BRAIN; PURIFICATION; INHIBITORS; LIVER; IDENTIFICATION; TISSUE; DIACYLGLYCEROL;
Keywords:
cannabinoid; anandamide; 2-arachidonoylglycerol; monoacylglycerol lipase; porcine brain; methyl arachidonyl fluorophosphonate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Yamamoto, S Univanokushima, Dept Biochem, Sch Med, Kuramoto Cho, Tokushima7708503, Jap Univ Tokushima Kuramoto Cho Tokushima Japan 7708503 8503, Jap
Citazione:
S.K. Goparaju et al., "Enzymes of porcine brain hydrolyzing 2-arachidonoylglycerol, an endogenousligand of cannabinoid receptors", BIOCH PHARM, 57(4), 1999, pp. 417-423

Abstract

Anandamide and 2-arachidonoylglycerol (2-AG) are two endogenous ligands for the cannabinoid receptors, and their cannabimimetic activities are lost when they are hydrolyzed enzymatically. Cytosol and particulate fractions ofporcine brain exhibited a high 2-AG hydrolyzing activity of 100 nmol/min/mg protein. Most of the activity could be attributed to a monoacylglycerol lipase-like enzyme that did not hydrolyze anandamide. It was separated by hydroxyapatite chromatography from anandamide amidohydrolase, which is also capable of hydrolyzing 2-AG as well as anandamide. Thus, porcine brain has at least two enzymes capable of hydrolyzing 2-AG. The 2-AG hydrolase activities of both the cytosolic and particulate enzymes were irreversibly and time-dependently inhibited by methyl arachidonyl fluorophosphonate with IC50 values as low as 2-3 nM. (C) 1999 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/02/20 alle ore 07:53:41