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Titolo:
Liquid chromatography microspray mass spectrometry for bacterial investigations
Autore:
Krishnamurthy, T; Davis, MT; Stahl, DC; Lee, TD;
Indirizzi:
USA,USAT Directorate, Edgewood RD&E Ctr, Aberdeen Proving Ground, MD 21010USA Aberdeen Proving Ground MD USA 21010 berdeen Proving Ground, MD 21010 City Hope Natl Med Ctr, Beckman Res Inst, Duarte, CA 91010 USA City Hope Natl Med Ctr Duarte CA USA 91010 Res Inst, Duarte, CA 91010 USA
Titolo Testata:
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
fascicolo: 1, volume: 13, anno: 1999,
pagine: 39 - 49
SICI:
0951-4198(1999)13:1<39:LCMMSF>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASSISTED-LASER-DESORPTION/IONIZATION; CHAIN-REACTION PRODUCTS; RAPID IDENTIFICATION; ENZYMATIC AMPLIFICATION; DNA-POLYMERASE; ELECTROSPRAY; DESORPTION; MICROORGANISMS; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Krishnamurthy, T USA,USAT Directorate, Edgewood RD&E Ctr, Aberdeen ProvingGround, MD 21010 USA Aberdeen Proving Ground MD USA 21010 round, MD 21010
Citazione:
T. Krishnamurthy et al., "Liquid chromatography microspray mass spectrometry for bacterial investigations", RAP C MASS, 13(1), 1999, pp. 39-49

Abstract

Cellular proteins (biomarkers) specific to any individual microorganism, determined by the direct mass spectral analysis of the corresponding intact cellular suspension, can be applied for the rapid and specific identification of the organisms present in unknown samples. The components of the bacterial suspensions, after a rapid separation over a Cls reversed-phase microcapillary column, were directly subjected to on-line electrospray ionizationfollowed by analysis using an ion trap tandem mass spectrometer, This approach is equally effective for gram-positive as well as gram-negative bacteria but has a distinct advantage over our earlier reported method involving matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). During electrospray ionitation mass spectrometry (ESI-MS),liquid samples can be directly analyzed and there is the potential for developing tandem mass spectral methods for more specific identification of the individual organisms present in crude bacterial mixtures. The total analysis time leading to unambiguous bacterial identification in samples was less than 10 minutes and the results were quite reproducible. Miniaturization of the instrumentation along with total automation of this simple process could have immense impact on field operations. Routine, rapid, cost-effective field monitoring of environmental samples, agricultural products, samplesfrom food processing, industrial sites and health institutions for suspected bacterial contamination could be a reality in the near future. Potentialutility in biological, medical, bioprocessing, pharmaceutical, and other industrial research is also enormous. Copyright (C) 1999 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 05:13:31